| Literature DB >> 32077696 |
Chang Liu1,2, Xiaolong Cui1,2, Boxuan Simen Zhao3, Pradnya Narkhede4, Yawei Gao5, Jun Liu1,2, Xiaoyang Dou1,2, Qing Dai1,2, Li-Sheng Zhang1,2, Chuan He1,2.
Abstract
DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.Entities:
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Year: 2020 PMID： 32077696 PMCID： PMC7216556 DOI： 10.1021/jacs.9b12707
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419