Literature DB >> 32070609

Tracking glycosylation in live cells using FTIR spectroscopy.

Joshua Phelan1, Ali Altharawi2, K L Andrew Chan3.   

Abstract

This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.
Copyright © 2020 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  ATR; Alkyne; Azide; FT-IR; Live cells; Metabolic labelling; Vibrational spectroscopy

Mesh：

Substances：

Year:  2020        PMID： 32070609      PMCID： PMC7048185          DOI： 10.1016/j.talanta.2020.120737

Source DB:  PubMed          Journal:  Talanta        ISSN： 0039-9140            Impact factor:   6.057


  28 in total

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