| Literature DB >> 32066961 |
Helen Farrants1,2, Miroslaw Tarnawski3, Thorsten G Müller4, Shotaro Otsuka5,6, Julien Hiblot1, Birgit Koch1, Moritz Kueblbeck5, Hans-Georg Kräusslich4, Jan Ellenberg5, Kai Johnsson7,8.
Abstract
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.Mesh：
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Year: 2020 PMID： 32066961 DOI： 10.1038/s41592-020-0746-7
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547