| Literature DB >> 32066955 |
Marta Derecka1, Josip Stefan Herman2,3,4, Pierre Cauchy2, Senthilkumar Ramamoorthy2, Ekaterina Lupar2, Dominic Grün2,5, Rudolf Grosschedl6.
Abstract
Crosstalk between mesenchymal stromal cells (MSCs) and hematopoietic stem cells (HSCs) is essential for hematopoietic homeostasis and lineage output. Here, we investigate how transcriptional changes in bone marrow (BM) MSCs result in long-lasting effects on HSCs. Single-cell analysis of Cxcl12-abundant reticular (CAR) cells and PDGFRα+Sca1+ (PαS) cells revealed an extensive cellular heterogeneity but uniform expression of the transcription factor gene Ebf1. Conditional deletion of Ebf1 in these MSCs altered their cellular composition, chromatin structure and gene expression profiles, including the reduced expression of adhesion-related genes. Functionally, the stromal-specific Ebf1 inactivation results in impaired adhesion of HSCs, leading to reduced quiescence and diminished myeloid output. Most notably, HSCs residing in the Ebf1-deficient niche underwent changes in their cellular composition and chromatin structure that persist in serial transplantations. Thus, genetic alterations in the BM niche lead to long-term functional changes of HSCs.Entities:
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Year: 2020 PMID： 32066955 DOI： 10.1038/s41590-020-0595-7
Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 25.606