Literature DB >> 32065188

The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols.

Jeremy David Adams1, Joachim Justad Røise, David Sam Lee, Niren Murthy.   

Abstract

We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.

Entities:  

Mesh：

Substances：

Year:  2020        PMID： 32065188      PMCID： PMC7886004          DOI： 10.1039/c9cc09136j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  18 in total

1.  Role of tyrosine 114 of L-methionine gamma-lyase from Pseudomonas putida.

Authors:  H Inoue; K Inagaki; N Adachi; T Tamura; N Esaki; K Soda; H Tanaka
Journal:  Biosci Biotechnol Biochem       Date:  2000-11       Impact factor: 2.043

2.  Tissue sulfhydryl groups.

Authors:  G L ELLMAN
Journal:  Arch Biochem Biophys       Date:  1959-05       Impact factor: 4.013

3.  A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity.

Authors:  Peng Qi; Dun Zhang; Yi Wan
Journal:  Talanta       Date:  2014-06-02       Impact factor: 6.057

4.  Approaches towards molecular amplification for sensing.

Authors:  Sean Goggins; Christopher G Frost
Journal:  Analyst       Date:  2016-04-27       Impact factor: 4.616

5.  An Auto-Inductive Cascade for the Optical Sensing of Thiols in Aqueous Media: Application in the Detection of a VX Nerve Agent Mimic.

Authors:  Xiaolong Sun; Eric V Anslyn
Journal:  Angew Chem Int Ed Engl       Date:  2017-07-03       Impact factor: 15.336

6.  The role of amino acid residues in the active site of L-methionine γ-lyase from Pseudomonas putida.

Authors:  Mitsuki Fukumoto; Daizou Kudou; Shouko Murano; Tomoo Shiba; Dan Sato; Takashi Tamura; Shigeharu Harada; Kenji Inagaki
Journal:  Biosci Biotechnol Biochem       Date:  2012-07-07       Impact factor: 2.043

7.  Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathway.

Authors:  Aymeric Goyer; Eva Collakova; Yair Shachar-Hill; Andrew D Hanson
Journal:  Plant Cell Physiol       Date:  2006-12-13       Impact factor: 4.927

8.  An Enzyme-Mediated Amplification Strategy Enables Detection of β-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β-Lactam Resistance.

Authors:  Tara R deBoer; Nicole J Tarlton; Reina Yamaji; Sheila Adams-Sapper; Tiffany Z Wu; Santanu Maity; Giri K Vesgesna; Corinne M Sadlowski; Peter DePaola; Lee W Riley; Niren Murthy
Journal:  Chembiochem       Date:  2018-09-26       Impact factor: 3.164

9.  Rates of thiol-disulfide interchange reactions involving proteins and kinetic measurements of thiol pKa values.

Authors:  Z Shaked; R P Szajewski; G M Whitesides
Journal:  Biochemistry       Date:  1980-09-02       Impact factor: 3.162

10.  Direct detection of the hydrolysis of nerve agent model compounds using a fluorescent probe.

Authors:  Xueying Zheng; Karl Okolotowicz; Beilin Wang; Mary Macdonald; John R Cashman; Jun Zhang
Journal:  Chem Biol Interact       Date:  2010-01-25       Impact factor: 5.192
View more
  2 in total

1.  A self-immolative linker that releases thiols detects penicillin amidase and nitroreductase with high sensitivity via absorption spectroscopy.

Authors:  Eli M Espinoza; Joachim J Røise; Maomao He; I-Che Li; Alvin K Agatep; Patrick Udenyi; Hesong Han; Nicole Jackson; D Lucas Kerr; Dake Chen; Michael R Stentzel; Emily Ruan; Lee Riley; Niren Murthy
Journal:  Chem Commun (Camb)       Date:  2022-03-03       Impact factor: 6.065

2.  Exponential amplification by redox cross-catalysis and unmasking of doubly protected molecular probes.

Authors:  Justine Pallu; Charlie Rabin; Pan Hui; Thamires S Moreira; Geordie Creste; Corentin Calvet; Benoît Limoges; François Mavré; Mathieu Branca
Journal:  Chem Sci       Date:  2022-02-11       Impact factor: 9.825
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.