Literature DB >> 30079487

An Enzyme-Mediated Amplification Strategy Enables Detection of β-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β-Lactam Resistance.

Tara R deBoer1, Nicole J Tarlton2, Reina Yamaji2, Sheila Adams-Sapper2, Tiffany Z Wu2, Santanu Maity1, Giri K Vesgesna1, Corinne M Sadlowski1, Peter DePaola1, Lee W Riley2, Niren Murthy1.   

Abstract

Biochemical assays that can identify β-lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current β-lactamase probes do not have the sensitivity needed to measure β-lactam resistance directly from patient samples. Here, we report the development of an instrument-free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of β-lactamase 40 000-fold, compared to the standard β-lactamase probe nitrocefin.
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  amplification; antimicrobial resistance; beta-lactamases; diagnostics; point-of-care diagnosis

Mesh：

Substances：

Year:  2018        PMID： 30079487      PMCID： PMC6494105          DOI： 10.1002/cbic.201800443

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  16 in total

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