Literature DB >> 320560

On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis.

C F Van Kreijl, R H Beelen, P Borst.   

Abstract

The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.

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Year:  1977        PMID： 320560      PMCID： PMC342444          DOI： 10.1093/nar/4.2.445

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

Review 1.  Recent advances in DNA sequence analysis.

Authors:  R Wu; R Bambara; E Jay
Journal:  CRC Crit Rev Biochem       Date:  1975-02

2.  Studies on polynucleotides. CXX. On the transcription of a synthetic 29-unit long deoxyribopolynucleotide.

Authors:  T Terao; J E Dahlberg; H G Khorana
Journal:  J Biol Chem       Date:  1972-10-10       Impact factor: 5.157

3.  Differential effects of sigma factor, ionic strength, and ribonucleoside triphosphate concentration on the transcription of phage T4 DNA with ribonucleic acid polymerase of Escherichia coli.

Authors:  D J Hoffman; S K Niyogi
Journal:  Biochim Biophys Acta       Date:  1973-04-11

4.  Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.

Authors:  E G Minkley; D Pribnow
Journal:  J Mol Biol       Date:  1973-06-25       Impact factor: 5.469

5.  The size of the repeating unit of the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.

Authors:  J N Mol; P Borst; F G Grosveld; J H Spencer
Journal:  Biochim Biophys Acta       Date:  1974-12-06

6.  Preparation of RNA transcripts of discrete segments of DNA.

Authors:  C A Marotta; P Lebowitz; R Dhar; B S Zain; S M Weissman
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

7.  Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.

Authors:  D Pribnow
Journal:  J Mol Biol       Date:  1975-12-15       Impact factor: 5.469

8.  Sequence of promoter for coat protein gene of bacteriophage fd.

Authors:  M Takanami; K Sugimoto; H Sugisaki; T Okamoto
Journal:  Nature       Date:  1976-03-25       Impact factor: 49.962

9.  RNA initiation with dinucleoside monophosphates during transcription of bacteriophage T4 DNA with RNA polymerase of Escherichia coli.

Authors:  D J Hoffman; S K Niyogi
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

10.  Studies on bacteriophage fd DNA. III. Nucleotide sequence preceding the RNA start-site on a promoter-containing fragment.

Authors:  K Sugimoto; H Sugisaki; T Okamoto; M Takanami
Journal:  Nucleic Acids Res       Date:  1975-11       Impact factor: 16.971
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  2 in total

1.  The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.

Authors:  C F Van Kreijl; J L Bos
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

2.  RNA sequencing with radioactive chain-terminating ribonucleotides.

Authors:  F R Kramer; D R Mills
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205
  2 in total

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