Sunil K Bose1, Monika Chauhan2, Neelima Dhingra2, Sanjay Chhibber1, Kusum Harjai1. 1. Department of Microbiology, BMS Block I, Panjab University, Chandigarh 160014, India. 2. University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India.
Abstract
Aim: To investigate the effects of Terpinen-4-ol on quorum sensing (QS)-regulated biofilm formation and virulence factors production in Pseudomonas aeruginosa. Materials & methods: QS inhibition, molecular docking analysis and gene expression studies were performed to check attenuation effect of Terpinen-4-ol on virulence of P. aeruginosa. Production of various virulence factors and biofilm formation were studied at sub-MIC of Terpinen-4-ol alone and in combination with ciprofloxacin. Results: Terpinen-4-ol at sub-MIC exhibited QS inhibition and downregulated all key QS genes. Molecular docking analysis showed high binding affinities of Terpinen-4-ol with QS receptors. Terpinen-4-ol exhibited synergistic interaction with ciprofloxacin and further reduced production of all the virulence factors and biofilms formation. Conclusion: Terpinen-4-ol could be developed into antivirulence drug after its in vivo evaluation for treatment strategies.
Aim: To investigate the effects of Terpinen-4-ol on quorum sensing (QS)-regulated biofilm formation and virulence factors production in Pseudomonas aeruginosa. Materials & methods: QS inhibition, molecular docking analysis and gene expression studies were performed to check attenuation effect of Terpinen-4-ol on virulence of P. aeruginosa. Production of various virulence factors and biofilm formation were studied at sub-MIC of Terpinen-4-ol alone and in combination with ciprofloxacin. Results:Terpinen-4-ol at sub-MIC exhibited QS inhibition and downregulated all key QS genes. Molecular docking analysis showed high binding affinities of Terpinen-4-ol with QS receptors. Terpinen-4-ol exhibited synergistic interaction with ciprofloxacin and further reduced production of all the virulence factors and biofilms formation. Conclusion:Terpinen-4-ol could be developed into antivirulence drug after its in vivo evaluation for treatment strategies.
Entities:
Keywords:
Pseudomonas aeruginosa; Terpinen-4-ol; antivirulence; biofilm; quorum sensing
Authors: María Constanza Luciardi; María Amparo Blázquez; María Rosa Alberto; Elena Cartagena; Mario Eduardo Arena Journal: Molecules Date: 2021-05-12 Impact factor: 4.411