Noriyoshi Sawabata1,2, Yoshiyuki Susaki3, Takahito Nakamura3, Takeshi Kawaguchi4, Motoaki Yasukawa4, Shigeki Taniguchi4. 1. Respiratory Disease Center, Hoshigaoka Medical Center, 4-8-1 Hoshigaoka, Hirakata City, Osaka, 573-8511, Japan. nsawabata@hotmail.com. 2. Department of Thoracic and Cardio-Vascular Surgery, Nara Medical University, 840 Shijo-cho, Kashihara City, Nara, 634-8522, Japan. nsawabata@hotmail.com. 3. Respiratory Disease Center, Hoshigaoka Medical Center, 4-8-1 Hoshigaoka, Hirakata City, Osaka, 573-8511, Japan. 4. Department of Thoracic and Cardio-Vascular Surgery, Nara Medical University, 840 Shijo-cho, Kashihara City, Nara, 634-8522, Japan.
Abstract
OBJECTIVES: A cancer lesion sheds tumor cells into the circulating blood as circulating tumor cells (CTCs). Since cluster CTCs have been considered as precursor lesions of metastasis, their clinical implication was investigated in this study according to the preoperative status of cluster CTC detection in surgical cases of clinically early-stage lung cancer. METHODS: Among 104 surgical patients of early-stage lung cancer, CTCs were extracted from the peripheral blood before surgery using a micro-pore size selection method (ScreenCell®) and diagnosed microscopically. Implications of detecting cluster CTC were assessed according to the prognosis and clinicopathological characteristics. RESULTS: The status of CTC detection was not detected in 77 cases (74.0%), single CTC only detection in 7 cases (6.7%), and cluster CTC detected in 20 cases (19.2%). Patients with cluster CTCs exhibited significantly lower recurrence-free survival and overall survival than did patients of other groups. In addition, in hazard ratio analysis, the hazard ratios were independent of other predictors of poor prognosis, and detection of cluster CTCs was associated with predictors of poor prognosis. CONCLUSION: Cluster CTCs were detected in cases where the original lung cancer lesion had clinical predictors of poor prognosis and were independent negative predictors of survival.
OBJECTIVES: A cancer lesion sheds tumor cells into the circulating blood as circulating tumor cells (CTCs). Since cluster CTCs have been considered as precursor lesions of metastasis, their clinical implication was investigated in this study according to the preoperative status of cluster CTC detection in surgical cases of clinically early-stage lung cancer. METHODS: Among 104 surgical patients of early-stage lung cancer, CTCs were extracted from the peripheral blood before surgery using a micro-pore size selection method (ScreenCell®) and diagnosed microscopically. Implications of detecting cluster CTC were assessed according to the prognosis and clinicopathological characteristics. RESULTS: The status of CTC detection was not detected in 77 cases (74.0%), single CTC only detection in 7 cases (6.7%), and cluster CTC detected in 20 cases (19.2%). Patients with cluster CTCs exhibited significantly lower recurrence-free survival and overall survival than did patients of other groups. In addition, in hazard ratio analysis, the hazard ratios were independent of other predictors of poor prognosis, and detection of cluster CTCs was associated with predictors of poor prognosis. CONCLUSION: Cluster CTCs were detected in cases where the original lung cancer lesion had clinical predictors of poor prognosis and were independent negative predictors of survival.
Entities:
Keywords:
Circulating tumor cell; Lung cancer; Surgery
Authors: Lauren Brady; Brian Hayes; Gráinne Sheill; Anne-Marie Baird; Emer Guinan; Bryan Stanfill; Tatjana Vlajnic; Orla Casey; Verena Murphy; John Greene; Emma H Allott; Juliette Hussey; Fidelma Cahill; Mieke Van Hemelrijck; Nicola Peat; Lorelei Mucci; Moya Cunningham; Liam Grogan; Thomas Lynch; Rustom P Manecksha; John McCaffrey; Dearbhaile O'Donnell; Orla Sheils; John O'Leary; Sarah Rudman; Ray McDermott; Stephen Finn Journal: PLoS One Date: 2020-12-18 Impact factor: 3.240