Literature DB >> 32041710

Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene tet(X) Variants from Bacterial, Fecal, and Environmental Samples.

Yulin Fu1, Dejun Liu1, Huangwei Song1, Zhihai Liu1,2, Haiyang Jiang3, Yang Wang3,4.   

Abstract

We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1) and tet(X2) and high-level tigecycline resistance genes tet(X3), tet(X4), and tet(X5). We showed that the real-time PCR assay developed had high linearity (R 2 ≥ 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples.
Copyright © 2020 American Society for Microbiology.

Entities:  

Keywords:  SYBR green; real-time PCR; tet(X); tigecycline resistance

Mesh：

Substances：

Year:  2020        PMID： 32041710      PMCID： PMC7179268          DOI： 10.1128/AAC.02292-19

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  17 in total

Review 1.  Tigecycline: what is it, and where should it be used?

Authors:  David M Livermore
Journal:  J Antimicrob Chemother       Date:  2005-08-24       Impact factor: 5.790

2.  Complete Nucleotide Sequence of a Novel Plasmid Bearing the High-Level Tigecycline Resistance Gene tet(X4).

Authors:  Liang-Xing Fang; Chong Chen; Dong-Ling Yu; Ruan-Yang Sun; Chao-Yue Cui; Liang Chen; Xiao-Ping Liao; Ya-Hong Liu; Jian Sun
Journal:  Antimicrob Agents Chemother       Date:  2019-10-22       Impact factor: 5.191

3.  Synthesis and structure-activity relationship of novel glycylcycline derivatives leading to the discovery of GAR-936.

Authors:  P E Sum; P Petersen
Journal:  Bioorg Med Chem Lett       Date:  1999-05-17       Impact factor: 2.823

Review 4.  Tetracycline Antibiotics and Resistance.

Authors:  Trudy H Grossman
Journal:  Cold Spring Harb Perspect Med       Date:  2016-04-01       Impact factor: 6.915

5.  Dissemination of antibiotic resistance genes and their potential removal by on-farm treatment processes in nine swine feedlots in Shandong Province, China.

Authors:  Weiwei Ben; Jian Wang; Xun Pan; Zhimin Qiang
Journal:  Chemosphere       Date:  2016-10-08       Impact factor: 7.086

6.  Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study.

Authors:  Karthikeyan K Kumarasamy; Mark A Toleman; Timothy R Walsh; Jay Bagaria; Fafhana Butt; Ravikumar Balakrishnan; Uma Chaudhary; Michel Doumith; Christian G Giske; Seema Irfan; Padma Krishnan; Anil V Kumar; Sunil Maharjan; Shazad Mushtaq; Tabassum Noorie; David L Paterson; Andrew Pearson; Claire Perry; Rachel Pike; Bhargavi Rao; Ujjwayini Ray; Jayanta B Sarma; Madhu Sharma; Elizabeth Sheridan; Mandayam A Thirunarayan; Jane Turton; Supriya Upadhyay; Marina Warner; William Welfare; David M Livermore; Neil Woodford
Journal:  Lancet Infect Dis       Date:  2010-08-10       Impact factor: 25.071

Review 7.  A review of tigecycline--the first glycylcycline.

Authors:  Lance R Peterson
Journal:  Int J Antimicrob Agents       Date:  2008-12       Impact factor: 5.283

8.  Emergence of mobile tigecycline resistance mechanism in Escherichia coli strains from migratory birds in China.

Authors:  Chong Chen; Chao-Yue Cui; Yan Zhang; Qian He; Xiao-Ting Wu; Gong Li; Xiao-Ping Liao; Barry N Kreiswirth; Ya-Hong Liu; Liang Chen; Jian Sun
Journal:  Emerg Microbes Infect       Date:  2019       Impact factor: 7.163

9.  Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli.

Authors:  Jian Sun; Chong Chen; Chao-Yue Cui; Yan Zhang; Xiao Liu; Ze-Hua Cui; Xiao-Yu Ma; Youjun Feng; Liang-Xing Fang; Xin-Lei Lian; Rong-Min Zhang; You-Zhi Tang; Kou-Xing Zhang; Han-Mian Liu; Zhi-Hui Zhuang; Shi-Dan Zhou; Jing-Nan Lv; Hong Du; Bin Huang; Fang-You Yu; Barun Mathema; Barry N Kreiswirth; Xiao-Ping Liao; Liang Chen; Ya-Hong Liu
Journal:  Nat Microbiol       Date:  2019-06-24       Impact factor: 17.745

10.  Prevalence and Abundance of Florfenicol and Linezolid Resistance Genes in Soils Adjacent to Swine Feedlots.

Authors:  Qin Zhao; Yang Wang; Shaolin Wang; Zheng Wang; Xiang-Dang Du; Haiyang Jiang; Xi Xia; Zhangqi Shen; Shuangyang Ding; Congming Wu; Bingrui Zhou; Yongning Wu; Jianzhong Shen
Journal:  Sci Rep       Date:  2016-08-30       Impact factor: 4.379
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  2 in total

1.  A TaqMan-based multiplex real-time PCR assay for the rapid detection of tigecycline resistance genes from bacteria, faeces and environmental samples.

Authors:  Yiming Li; Zhangqi Shen; Shuangyang Ding; Shaolin Wang
Journal:  BMC Microbiol       Date:  2020-06-22       Impact factor: 3.605

2.  Rapid Detection of High-Level Tigecycline Resistance in Tet(X)-Producing Escherichia coli and Acinetobacter spp. Based on MALDI-TOF MS.

Authors:  Ze-Hua Cui; Zi-Jian Zheng; Tian Tang; Zi-Xing Zhong; Chao-Yue Cui; Xin-Lei Lian; Liang-Xing Fang; Qian He; Xi-Ran Wang; Chong Chen; Bing He; Min-Ge Wang; Ya-Hong Liu; Xiao-Ping Liao; Jian Sun
Journal:  Front Cell Infect Microbiol       Date:  2020-09-25       Impact factor: 5.293
  2 in total

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