| Literature DB >> 32038774 |
Jae-Hee Hyun1, Jung-Il Kang1, Sang-Cheol Kim1, Elvira Kim1, Ji-Hoon Kang1, Jung-Mi Kwon1, Doek-Bae Park1, Young-Jae Lee2, Eun-Sook Yoo1, Hee-Kyoung Kang1.
Abstract
The present study investigated the anti-proliferative and chemosensitizing effects of Crinum asiaticum var. japonicum against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform (CHCI3) fraction and butanol (BuOH) fraction of C asiaticum inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the CHCI3 and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced Bcl-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The CHCI3 fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the CHCI3 fraction of C asiaticum plays a pivotal role as a chemosensitizer. We suggest that components of C asiaticum might have a therapeutic potential for the treatment of MDR leukemia. © Korean Society of Toxicology 2008.Entities:
Keywords: Apoptosis; BCRR; Chemosensitizer; Crinum asiaticum; HL-60/MX2; MRP-1
Year: 2008 PMID： 32038774 PMCID： PMC7006299 DOI： 10.5487/TR.2008.24.1.029
Source DB: PubMed Journal: Toxicol Res ISSN： 1976-8257