Chun-Kai Fu1,2,3, Yi-Chun Chien4, Hui-Yen Chuang5, Yun-Chi Wang3, Jeng-Jong Hwang5,6, Mei-Due Yang3, Chien-Chih Yu3, Jaw-Chyun Chen7, Wen-Shin Chang8, DA-Tian Bau9,3,10, Chia-Wen Tsai8. 1. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. 2. Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C. 3. Terry Fox Cancer Research Laboratory, Translational Medicine Center, China Medical University Hospital, Taichung, Taiwan, R.O.C. 4. Department of Medical Imaging and Radiological Sciences, I-Shou University, Jiaosu Village, Kaohsiung, Taiwan, R.O.C. 5. Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan, R.O.C. 6. Department of Medical Imaging, Chung Shan Medical University Hospital, Taichung, Taiwan, R.O.C. 7. Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan, R.O.C. 8. Terry Fox Cancer Research Laboratory, Translational Medicine Center, China Medical University Hospital, Taichung, Taiwan, R.O.C. artbau2@gmail.com wenwen816@gmail.com halittlemelon@hotmail.com. 9. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. artbau2@gmail.com wenwen816@gmail.com halittlemelon@hotmail.com. 10. Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Few studies have examined the genetic role of matrix metalloproteinases (MMPs) to early detection or prediction in gastric cancer development. In this study, the contribution of MMP7 promoter (A-181G and C-153T) polymorphic genotypes to gastric cancer risk in Taiwanese was investigated for the first time. MATERIALS AND METHODS: A total of 121 cases and 363 controls were enrolled and their MMP7 genotypes at A-181G and C-153T were examined by polymerase chain reaction-restriction fragment length polymorphism methodology using genomic DNA from serum. RESULTS: The GG genotype at MMP7 A-181G was found to represent a risk factor for gastric cancer, especially among smokers. No individual with variant genotype carrier at MMP7 C-153T was found among this Taiwanese population. CONCLUSION: The G allele of MMP7 A-181G may serve as an early predictor for gastric cancer risk in Taiwanese; other gastric cancer markers are still urgently needed. Copyright
BACKGROUND/AIM: Few studies have examined the genetic role of matrix metalloproteinases (MMPs) to early detection or prediction in gastric cancer development. In this study, the contribution of MMP7 promoter (A-181G and C-153T) polymorphic genotypes to gastric cancer risk in Taiwanese was investigated for the first time. MATERIALS AND METHODS: A total of 121 cases and 363 controls were enrolled and their MMP7 genotypes at A-181G and C-153T were examined by polymerase chain reaction-restriction fragment length polymorphism methodology using genomic DNA from serum. RESULTS: The GG genotype at MMP7A-181G was found to represent a risk factor for gastric cancer, especially among smokers. No individual with variant genotype carrier at MMP7C-153T was found among this Taiwanese population. CONCLUSION: The G allele of MMP7A-181G may serve as an early predictor for gastric cancer risk in Taiwanese; other gastric cancer markers are still urgently needed. Copyright