Sundaram Ramalingam1,2, Muthu Karuppiah3, Muthusamy Thiruppathi4, Shanthi Palanivelu5, Sachdanandam Panchanatham1. 1. Department of Medical Biochemistry, University of Madras, Chennai, India. 2. Department of Biochemistry, Saveetha Dental College & Hospital, Saveetha Institute of Medical &Technical Sciences, Saveetha University, Chennai, India. 3. Department of Chemistry, Manonmaniam Sundaranar University, Tirunelveli, India. 4. Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, IL, USA. 5. Department of Pathology, University of Madras, Chennai, India.
Abstract
Objectives: The present study was to isolate the biflavonoid (a bimolecular kaemferol structured molecule) and test its efficacy on oxidative stress and carbohydrate metabolic key enzymes in control and high fat diet and streptozotocin -induced diabetic rats. Methods: Type 2 diabetes was induced in male albino wistar rats by feeding them with high fat diet comprising of 84.3% standard laboratory chow, 5% lard, 10% yolk powder, cholesterol 0.2%, and 0.5% bile salt for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (35 mg/kg, dissolved in 0.1 M sodium citrate buffer, pH 4.5). Results: At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabetic rats.Discussion: Oral administration of biflavonoid to diabetic rats significantly ameliorated all the biochemical alterations to near normal levels. The effect produced by the biflavonoid on various parameters was comparable to that of metformin.
Objectives: The present study was to isolate the biflavonoid (a bimolecular kaemferol structured molecule) and test its efficacy on oxidative stress and carbohydrate metabolic key enzymes in control and high fat diet and streptozotocin -induced diabeticrats. Methods: Type 2 diabetes was induced in male albino wistar rats by feeding them with high fat diet comprising of 84.3% standard laboratory chow, 5% lard, 10% yolk powder, cholesterol 0.2%, and 0.5% bile salt for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (35 mg/kg, dissolved in 0.1 M sodium citrate buffer, pH 4.5). Results: At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabeticrats.Discussion: Oral administration of biflavonoid to diabeticrats significantly ameliorated all the biochemical alterations to near normal levels. The effect produced by the biflavonoid on various parameters was comparable to that of metformin.
Authors: G Ramírez-Venegas; D L De Ita-Pérez; M Díaz-Muñoz; I Méndez; T García-Gasca; M Ahumada-Solórzano; X Zambrano-Estrada; O Vázquez-Martínez; H Guzmán-Maldonado; D Luna-Moreno Journal: Plant Foods Hum Nutr Date: 2021-07-04 Impact factor: 3.921