A Fixed-Dose Combination Of Gemigliptin And Rosuvastatin Exhibits Similar Pharmacokinetics, Pharmacodynamics, And Safety Compared To That Of A Loose Combination In Healthy Subjects.

PURPOSE: Fixed-dose combination (FDC) of gemigliptin and rosuvastatin may improve medication compliance of patients with comorbid type 2 diabetes and dyslipidemia. Pharmacokinetics (PK), pharmacodynamics (PD), and safety of gemigliptin/rosuvastatin 50/20 mg FDC was compared with a loose combination of individual tablets in healthy subjects. PATIENTS AND METHODS: A randomized, open-label, single-dose, two-period, two-sequence, two-treatment crossover study was conducted. Subjects received FDC or a loose combination of gemigliptin (50 mg) and rosuvastatin (20 mg) during each period, with a 14-day washout. Serial blood samples were collected up to 72 hrs after dosing to measure plasma concentrations of gemigliptin, its active metabolite LC15-0636, and rosuvastatin for PK assessment, and DPP-4 activity for PD assessment. PK and PD parameters were calculated using a non-compartmental method. Safety profiles were evaluated throughout the study.
RESULTS: Thirty-seven subjects completed the study. The concentration-time profiles of gemigliptin, LC15-0636, and rosuvastatin were similar between FDC and loose combination, respectively. For each of the three compounds, the geometric mean ratios (90% confidence interval) of FDC to loose combination for Cmax and AUClast fell within the bioequivalence range of 0.8-1.25. Inhibition of DPP-4 activity-time profiles after administration of FDC and loose combination was overlapping, and Imax and AUEClast were similar. Both FDC and the loose combination were well tolerated.
CONCLUSION: PK, PD, and safety profiles of gemigliptin, its metabolite, and rosuvastatin were similar between FDC and loose combination. The FDC of gemigliptin (50 mg) and rosuvastatin (20 mg) can be used as an alternative to a loose combination, which is expected to improve patient compliance.

Introduction

Patients with type 2 diabetes frequently suffer from dyslipidemia. According to a retrospective study, 77.2% of patients with type 2 diabetes had hyperlipidemia, which is the third highest disease comorbid with type 2 diabetes.1 In these patients, cardiovascular disease is the leading cause of death, and appropriate medication is needed to reduce the risk of cardiovascular disease.2,3 To reduce the economic burden and mortality of patients with type 2 diabetes and dyslipidemia, and to improve patient compliance, there is a need for the development of fixed-dose combinations (FDC) for treatment of both diseases.

DPP-4 inhibitors can be used as alternatives to metformin for treatment of mild hyperglycemia (A1C<7.5%), and are used as add-on drugs in combination with metformin in patients with A1C≥7.5%.4 Gemigliptin is a potent DPP-4 inhibitor and has been used as a treatment for type 2 diabetes since approval in 2012 by the Ministry of Food and Drug Safety (Republic of Korea).5,6 Gemigliptin is metabolized by cytochrome P450 (CYP), resulting in conversion to the metabolite LC15-0636, which has 2-fold higher DPP-4 inhibitory potency in vitro than the parent compound.7,8 Gemigliptin, like other DPP-4 inhibitors,4 is associated with a low risk of hypoglycemia and is neutral to body weight according to previous studies.9,10

For patients with dyslipidemia and type 2 diabetes requiring drug therapy to lower cholesterol,4 statins are recommended as a first-line drug therapy.4 Statins lower the risk of cardiovascular events and death in these patients.4 Rosuvastatin was effective in reducing low-density lipoprotein cholesterol (LDL-C) levels and was well tolerated in patients with dyslipidemia and type 2 diabetes.11–13 Rosuvastatin is excreted as its parent form in feces, with little hepatic metabolism.14

According to a previous study, pharmacokinetic properties of rosuvastatin are not altered by gemigliptin and vice versa.15 The maximum daily dose of gemigliptin approved by the Ministry of Food and Drug Safety (Republic of Korea) is 50 mg (Zemiglo®, LG Chem. 2017). The maximum daily dose of rosuvastatin is 20 mg in Asia (Crestor®, AstraZeneca. 2016. Republic of Korea) due to higher systemic exposure to rosuvastatin in Asian subjects compared to caucasian subjects.16,17 Both gemigliptin and rosuvastatin are administered once daily, regardless of diet.5,18 A new FDC of gemigliptin and rosuvastatin was developed and is expected to improve convenience of use and increase medication adherence in patients with type 2 diabetes and dyslipidemia.

The aim of this study was to investigate the pharmacokinetics (PK), pharmacodynamics (PD), and safety of gemigliptin (50 mg) and rosuvastatin (20 mg) when administered as an FDC compared to a loose combination in healthy subjects.

Materials And Methods

Study Design

This study was a randomized, open-label, single-dose, two-period, two-treatment, two-sequence crossover clinical trial. Subjects were randomly assigned to the two sequences at a ratio of 1:1. In each period, subjects were admitted to the hospital 1 day prior to the administration of the study drug and were fasted overnight. Subjects then received a loose combination of gemigliptin 50 mg (LG Chem., batch number GEM15518C) and rosuvastatin 20 mg (AstraZeneca, batch number 60019434) or an FDC of gemigliptin/rosuvastatin 50/20 mg (LG Chem., batch number 16GS81-1) with 200 mL of water in the first period. After a 14-day washout period, the subjects received an alternate treatment in the second period. Drinking water was prohibited for 1 hr before dosing and 2 hrs after dosing. Food intake was prohibited for 4 hrs after dosing.

Blood samples for PK and PD assessment were collected pre-dose and at 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 12, 24, 48, 72 hrs post-dose. Approximately 8 mL of blood for PK analysis and 3 mL of blood for PD analysis were taken at each blood sampling through a venous puncture or a saline-locked angiocatheter placed in the upper arm vein.

This study was conducted under the approval of the institutional review board of Seoul National University Hospital (SNUH), and the Korea Ministry of Food and Drug Safety (ClinicalTrials.gov registry number: NCT02670070). The study was conducted in accordance with the ethical guidelines of the Declaration of Helsinki and Good Clinical Practice of International Conference on Harmonization. Written informed consent was obtained from all subjects before conducting the screening test for participation in this study.

Subjects And Treatments

Healthy male subjects aged 19–45 years with a body mass index of 18–27 kg/m2 and fasting glucose of 70–125 mg/dL at screening were enrolled in this study. Subjects who had clinically significant abnormalities during physical examination, electrocardiogram monitoring test, clinical laboratory tests, or vital signs at screening were excluded. In addition, subjects who had hypersensitivity reactions to gemigliptin or rosuvastatin and who had a history of drug-induced muscle disease were excluded.

Sample size was calculated based on prior study results, from which the intra-subject coefficient of variation of the major pharmacokinetic parameters was assumed to be about 20% for gemigliptin and about 30% for rosuvastatin. Assuming a dropout rate of 20%, inclusion of 40 subjects was estimated to result in 80% power at a significance level of 0.05 to detect whether the 90% confidence interval (90% CI) for comparison of pharmacokinetic parameters of FDC to loose combination was within the bioequivalence range of 0.8–1.25.19

Determination Of Plasma Drug Concentration And DPP-4 Activity

Blood samples for PK assessment were collected in heparinized tubes and centrifuged at 1,800 rpm for 8 mins at 4°C. After centrifugation, plasma samples for gemigliptin analysis were transferred to tubes containing an equal amount of 5% formic acid solution for long-term stability of gemigliptin.8 Plasma samples for rosuvastatin analysis were placed in tubes after centrifugation without any additional treatment. Blood samples for PD assessment were collected in EDTA tubes and centrifuged. All plasma samples for PK and PD assessment were placed in a −20 °C freezer immediately after pretreatment (if necessary) and stored at −70°C within 24 hrs until sample analysis.

Plasma concentrations of gemigliptin, LC15-0636, and rosuvastatin were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS; Shiseido NASCA and AB Sciex Triple Quad 5500). Gemigliptin, LC15-0636, and rosuvastatin were separated using a UK-C18 column (3 μm, 50 x 2 mm, Imatackt, Japan), and detected by positive electrospray ionization with multiple reaction monitoring (AB Sciex Triple Quad 5500).

The analytical method was validated across the range of 0.5–200 ng/mL for gemigliptin, and 0.25–100 ng/mL for LC15-0636. For gemigliptin, the mean accuracies of the quality control (QC) samples were within 103–105% and the coefficient of variation (CV) was ≤4.46%. For LC15-0636, the corresponding values were 102–104% and ≤6.33%, respectively. The analytical method was validated across the range of 0.25–100 ng/mL for rosuvastatin, and the mean accuracies of the quality control samples were within 90.7–101% and the CV was ≤5.88%. All samples of FDC and loose combination were analyzed according to the validated method as described above.

Plasma DPP-4 activity was determined using a continuous spectrophotometric assay as described in a previous study.20

Pharmacokinetic/pharmacodynamic Assessment

Pharmacokinetic (PK) and pharmacodynamic (PD) assessments were performed on subjects who completed all planned PK and PD blood sampling. Calculation of PK and PD parameters was performed using Phoenix® WinNonlin® software, version 7.0 (Certara USA Inc., Princeton, NJ, USA) using the non-compartment method. For PK assessment, area under the concentration–time curve from 0 to the last measurable concentration (AUClast) and maximum concentration (Cmax) were calculated. For PD assessment, the area under the inhibition of DPP activity–time curve from 0 to the last measurable concentration (AUEClast) and maximum inhibition of DPP-4 activity (Imax) were calculated using change from the basal value. All statistical analyses were performed using SAS version 9.4 (SAS Institute Inc., Cary, NC, USA). The PK and PD parameters were log-transformed and the geometric mean ratio (GMR) of FDC to loose combination and its 90% CIs were estimated using a linear mixed-effect model. The model contained period, sequence, and treatment as fixed effects, and subject nested within sequence as a random effect. It was concluded that there were no significant PK differences between the two treatments when GMR (90% CI) of FDC to loose combination was contained within the bioequivalence range of 0.8–1.25.

Safety Assessment

Safety evaluation was performed on subjects who received the study drugs at least once. All subjects were examined for adverse events (AEs), physical examination, electrocardiogram test, clinical laboratory tests, and vital signs. The results of each assessment were reviewed to determine their clinical significance and to determine whether they were related to study drugs.

Results

Study Population

A total of 42 subjects were enrolled in the study, two of whom were withdrawn due to abnormal ECG test results before the first administration. Forty subjects received the study drug at least once and these subjects were evaluated for demographic characteristics and safety. After the first administration and before the second administration, two subjects were withdrawn due to withdrawal of consent and use of concomitant medication without investigator approval. Another subject was dropped due to an adverse event (gastroenteritis) which was unlikely related to study drug. A total of 37 subjects completed the study and these subjects were evaluated for PK and PD. Demographic characteristics are summarized in Table 1. There were no significant differences in subject characteristics between the two sequences (Table 1).

Table 1

Demographic Characteristics Of The Subjects

	Sequence A (N = 20)	Sequence B (N = 20)	p-value
Age (years)	28.5 ± 5.3 (21–42)	28.1 ± 5.6 (21–44)	0.6640*
Height (cm)	176.4 ± 7.0 (165–186)	173.5 ± 5.3 (161–180)	0.1753*
Weight (kg)	72.3 ± 9.2 (56.2–87.2)	68.8 ± 7.3 (53.0–85.6)	0.1996**
BMI (kg/m2)	23.2 ± 2.3 (18.5–26.9)	22.9 ± 2.1 (18.3–27.0)	0.6103**

Notes: Data are presented as arithmetic mean ± standard deviation. (min-max). Sequence A was first administered the loose combination of gemigliptin (50 mg) and rosuvastatin (20 mg), followed by the fixed-dose combination of gemigliptin/rosuvastatin 50/20 mg, and sequence B was administered in the reverse order. * P-value was obtained from Mann–Whitney U-test. ** P-value was obtained from two-sample t-test.

Pharmacokinetic/pharmacodynamic Results

Plasma concentration-time profiles and PK parameters of gemigliptin, LC15-0636, and rosuvastatin after administration of FDC and loose combination were superimposed (Figure 1). The GMR (90% CI) of gemigliptin Cmax and AUClast for FDC to loose combination was 1.08 (0.99–1.18) and 1.02 (0.96–1.09), respectively. The GMR (90% CI) of rosuvastatin Cmax and AUClast for FDC to loose combination was 1.09 (0.96–1.22) and 1.02 (0.95–1.09), respectively. In addition, the corresponding values of LC15-0636 were 1.00 (0.93–1.08) and 1.02 (0.97–1.08). Overall, the GMR (90% CI) of the PK parameters of gemigliptin and rosuvastatin fell within the bioequivalence criteria of 0.8–1.25, which shows that systemic exposure is similar between the two treatments (Table 2).

Figure 1

Mean plasma concentration-time profiles of (A) gemigliptin and LC15-0636 and (B) rosuvastatin after a single administration of a fixed-dose combination and loose combination of gemigliptin 50 mg and rosuvastatin 20 mg. Error bars represent standard deviations.

Table 2

Pharmacokinetic Parameters Of Rosuvastatin, Gemigliptin, And LC15-0636 After Administration Of Gemigliptin (50 mg) And Rosuvastatin (20 mg) As A Fixed-Dose Combination Or Loose Combination

Parameter	Rosuvastatin			Gemigliptin			LC15-0636
FDCa(N = 37)	LCb(N = 37)	Geometric Mean Ratio*(90% CI)	FDCa(N = 37)	LCb(N = 37)	Geometric mean Ratio*(90% CI)	FDCa(N = 37)	LCb(N = 37)	Geometric Mean Ratio*(90% CI)
Tmax (h)	4.00 [1.50–8.00]	4.00 [0.50–6.02]	-	1.50 [0.50–5.02]	1.50 [0.50–5.00]	-	4.00 [1.00–5.02]	5.00 [1.00–6.00]	-
Cmax (μg/L)	27.85 ± 10.68	26.59 ± 12.20	1.0857 (0.9647–1.2218)	71.89 ± 22.59	66.29 ± 21.51	1.0791 (0.9869–1.1800)	10.70 ± 2.34	10.68 ± 2.45	1.0038 (0.9344–1.0785)
AUClast (h·μg/L)	285.78 ± 96.14	280.39 ± 94.42	1.0184 (0.9530–1.0882)	878.37 ± 217.67	842.40 ± 121.46	1.0233 (0.9605–1.0901)	278.03 ± 54.83	268.70 ± 36.83	1.0248 (0.9749–1.0773)
AUCinf (h·μg/L)	295.89 ± 94.87	291.40 ± 94.61	1.0184 (0.9596–1.0807)	968.83 ± 423.31	894.85 ± 132.09	1.0358 (0.9510–1.1281)	353.24 ± 183.33	324.19 ± 47.06	1.0335(0.9512–1.1230)
t1/2 (h)	11.33 ± 2.59	12.63 ± 4.12	-	18.92 ± 6.79	18.45 ± 1.73	-	28.69 ± 12.17	28.06 ± 4.88	-
CL/F (L/h)	75.89 ± 29.82	77.29 ± 29.09	-	56.51 ± 14.82	57.09 ± 8.63	-	-	-	-
Vd/F (L)	1207.95 ± 437.89	1422.75 ± 796.43	-	1473.26 ± 359.25	1510.09 ± 209.17	-	-	-	-
Metabolic ratio**	-	-	-	-	-	-	0.33 ± 0.06	0.32 ± 0.06	-

Notes: Data are presented as arithmetic mean ± standard deviation (SD) except for Tmax, for which median [min–max] is presented. *Values expressed as geometric mean ratio (90% Confidence interval, CI) of FDC to loose combination. **Ratio of AUClast for metabolite (LC15-0636) to parent drug (gemigliptin). Values are presented as mean ± SD. aFDC: fixed-dose combination of gemigliptin/rosuvastatin 50/20 mg. bLC: loose combination of gemigliptin 50 mg and rosuvastatin 20 mg.

Abbreviations: AUCinf, area under the concentration–time curve from 0 to infinity; AUClast, area under the concentration–time curve from 0 to the last measurable timepoint; CI, confidence interval; CL/F, apparent clearance; Cmax, maximum concentration; GMR, geometric mean ratio of FDC to separate tablets; Tmax, time to reach the Cmax; t1/2, terminal half-life; and Vd/F, apparent volume of distribution after oral dosing.

DPP-4 activity inhibition-time profiles after administration of FDC and loose combination were superimposed (Figure 2). Inhibition of plasma DPP-4 activity following FDC and loose combination administration was similar when evaluated by AUEClast and Imax. The GMR (90% CI) of AUEClast and Imax for FDC to loose combination was 1.00 (0.98–1.03) and 1.01 (1.00–1.02), respectively (Table 3).

Figure 2

Mean plasma DPP-4 activity inhibition from baseline–time profiles after administration of a fixed-dose combination and loose combination of gemigliptin and rosuvastatin. Error bars represent standard deviations.

Table 3

Comparison Of Plasma DPP-4 Activity Inhibition From Baseline After Administration Of Gemigliptin (50 mg) And Rosuvastatin (20 mg) As A Fixed-Dose Combination Or Loose Combination

Parameter	DPP-4 Activity Inhibition (%)
	Fixed-Dose Combination	Loose Combination	Geometric Mean Ratio*
(N = 37)	(N = 37)	(90% CI)
AUEClast (%·h)	4838.76 ± 442.36	4826.99 ± 511.14	1.0041 (0.9797–1.0291)
Imax (%)	93.35 ± 2.58	92.65 ± 3.78	1.0082 (0.9956–1.0210)

Note: *Values expressed as geometric mean ratio (90% CI) of fixed-dose combination to loose combination. Data are presented as arithmetic mean ± standard deviation.

Abbreviations: AUEClast, area under the inhibition of DPP activity–time curve from 0 to the last measurable timepoint; CI, confidence interval; Imax, maximum inhibition of DPP-4 activity.

Safety Results

The incidence of drug-related AEs was similar between loose combination and FDC. A total of 2 drug-related AEs (neck pain and upper respiratory tract infection) occurred in 2 subjects after administration of loose combination, and 3 drug-related AEs (2 cases of oropharyngeal pain and 1 case of cough) occurred in 3 subjects after administration of FDC. The subject who had upper respiratory tract infection was administered xylometazoline HCl (Metarivin 0.1%) without investigator approval and was removed from the study because of the possibility of interaction between xylometazoline and the study drugs. All drug-related AEs were mild and there was no serious AE. In addition, all patients who experienced AEs successfully recovered and remained in the study. There were no clinically significant changes in physical examinations, electrocardiograms, clinical laboratory tests, or vital signs.

Discussion

This study was conducted to evaluate PK, PD, and safety of FDC of gemigliptin (50 mg) and rosuvastatin (20 mg) compared to loose combination. The GMR and 90% CI of Cmax and AUClast for gemigliptin, its metabolite LC15-0636, and rosuvastatin fell within the bioequivalence criteria of 0.8–1.25. The GMR and 90% CI of AUEC and Imax for DPP-4 were also within the range of 0.8–1.25. In addition, all AEs were mild, and the number of AEs and the number of subjects who developed AEs were similar for both formulations. Overall, the two formulations were found to be similar in PK, PD, and safety aspects. This suggests that FDC may be a new, convenient option for patients with type 2 diabetes and dyslipidemia.

The washout period of this study was 14 days, which is long enough to eliminate carryover effects considering previously reported half-lives of 17 h for gemigliptin, 27 h for LC15-0636, and 19 h for rosuvastatin.9,15,21 However, one case of carryover effect for gemigliptin and rosuvastatin was observed at pre-dose in the second period after washout. However, PK analysis was performed without any adjustments because both of gemigliptin and rosuvastatin concentrations were less than 5% of Cmax.19

In this study, DPP-4 inhibition by gemigliptin was evaluated as a PD parameter. DPP-4 inhibition increases glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) levels, thereby increasing insulin secretion and ultimately lowering glucose levels.22 However, PD parameters of rosuvastatin were not evaluated in this study because changes in cholesterol synthesis induced by rosuvastatin are not immediate as LDL-C is reduced by 64% in the first week after rosuvastatin administration and by 90% in the second week.23

Conclusion

PK, PD, and safety profiles of gemigliptin, its metabolite, and rosuvastatin were similar between FDC and loose combination. The FDC of gemigliptin (50 mg) and rosuvastatin (20 mg) can be used as an alternative to loose combination, which is expected to improve patient compliance.