Literature DB >> 3200835

Translational repression in eukaryotes: partial purification and characterization of a repressor of ferritin mRNA translation.

W E Walden1, S Daniels-McQueen, P H Brown, L Gaffield, D A Russell, D Bielser, L C Bailey, R E Thach.   

Abstract

Mouse and rabbit ferritin mRNAs translate very poorly in rabbit reticulocyte lysates relative to most other mRNAs. This translational deficiency is not seen in wheat germ lysates, suggesting the presence of an inhibitor in reticulocyte lysate that is specific for ferritin mRNA. A specific repressor of ferritin mRNA translation has been partially purified from rabbit reticulocytes by differential ultracentrifugation, ammonium sulfate fractionation, and chromatography on phosphocellulose, DEAE-cellulose, and Sephacryl S-300. The elution profile from the latter suggests an aggregate molecular mass of approximately 180 kDa for the repressor. The inhibitory activity of this repressor against native ferritin mRNA can be relieved by adding in vitro transcripts of ferritin light-chain RNAs that contain the first 92 nucleotides of the 5' untranslated region. No other sequences appear to be necessary for this effect.

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Year:  1988        PMID： 3200835      PMCID： PMC282781          DOI： 10.1073/pnas.85.24.9503

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Discriminatory interaction of purified eukaryotic initiation factors 4F plus 4A with the 5' ends of reovirus messenger RNAs.

Authors:  T G Lawson; M H Cladaras; B K Ray; K A Lee; R D Abramson; W C Merrick; R E Thach
Journal:  J Biol Chem       Date:  1988-05-25       Impact factor: 5.157

2.  The importance of the 3'-untranslated region in the translational control of ferritin mRNA.

Authors:  L F Dickey; Y H Wang; G E Shull; I A Wortman; E C Theil
Journal:  J Biol Chem       Date:  1988-03-05       Impact factor: 5.157

3.  Procedures for enhancing the utility of the metallothionein promoter for the regulated expression of downstream open reading frames.

Authors:  W E Walden; S Daniels-McQueen; L L Smith; R E Thach
Journal:  Gene       Date:  1987       Impact factor: 3.688

4.  Translational control of gene expression in a normal fibroblast. Characterization of a subclass of mRNAs with unusual kinetic properties.

Authors:  W E Walden; R E Thach
Journal:  Biochemistry       Date:  1986-04-22       Impact factor: 3.162

Review 5.  Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms.

Authors:  E C Theil
Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

6.  Identification of the iron-responsive element for the translational regulation of human ferritin mRNA.

Authors:  M W Hentze; S W Caughman; T A Rouault; J G Barriocanal; A Dancis; J B Harford; R D Klausner
Journal:  Science       Date:  1987-12-11       Impact factor: 47.728

7.  Iron regulates ferritin mRNA translation through a segment of its 5' untranslated region.

Authors:  N Aziz; H N Munro
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

8.  Conservation of ferritin heavy subunit gene structure: implications for the regulation of ferritin gene expression.

Authors:  M T Murray; K White; H N Munro
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

9.  Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation.

Authors:  J L Casey; M W Hentze; D M Koeller; S W Caughman; T A Rouault; R D Klausner; J B Harford
Journal:  Science       Date:  1988-05-13       Impact factor: 47.728

10.  Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.

Authors:  E A Leibold; H N Munro
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205
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  33 in total

1.  A high yield affinity purification method for specific RNA-binding proteins: isolation of the iron regulatory factor from human placenta.

Authors:  B Neupert; N A Thompson; C Meyer; L C Kühn
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

2.  Two hypervariable minisatellite DNA binding proteins.

Authors:  W P Wahls; G Swenson; P D Moore
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

3.  Cytoplasmic protein binding to highly conserved sequences in the 3' untranslated region of mouse protamine 2 mRNA, a translationally regulated transcript of male germ cells.

Authors:  Y K Kwon; N B Hecht
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-01       Impact factor: 11.205

4.  Position is the critical determinant for function of iron-responsive elements as translational regulators.

Authors:  B Goossen; M W Hentze
Journal:  Mol Cell Biol       Date:  1992-05       Impact factor: 4.272

5.  Modulation of ferritin H-chain expression in Friend erythroleukemia cells: transcriptional and translational regulation by hemin.

Authors:  E M Coccia; V Profita; G Fiorucci; G Romeo; E Affabris; U Testa; M W Hentze; A Battistini
Journal:  Mol Cell Biol       Date:  1992-07       Impact factor: 4.272

Review 6.  Mechanism and regulation of eukaryotic protein synthesis.

Authors:  W C Merrick
Journal:  Microbiol Rev       Date:  1992-06

7.  Ferritin mRNA: interactions of iron regulatory element with translational regulator protein P-90 and the effect on base-paired flanking regions.

Authors:  C M Harrell; A R McKenzie; M M Patino; W E Walden; E C Theil
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

8.  Regulation of ferritin and heme oxygenase synthesis in rat fibroblasts by different forms of iron.

Authors:  R S Eisenstein; D Garcia-Mayol; W Pettingell; H N Munro
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-01       Impact factor: 11.205

9.  Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.

Authors:  M T Murray; D L Schiller; W W Franke
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

10.  Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase.

Authors:  E Chu; D M Koeller; J L Casey; J C Drake; B A Chabner; P C Elwood; S Zinn; C J Allegra
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205
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