Literature DB >> 3200317

Enhanced protein thermostability from designed mutations that interact with alpha-helix dipoles.

H Nicholson1, W J Becktel, B W Matthews.   

Abstract

Two different genetically engineered amino-acid substitutions designed to interact with alpha-helix dipoles in T4 lysozyme are shown to increase the thermal stability of the protein. Crystallographic analyses of the mutant lysozyme structures suggest that the stabilization is due to electrostatic interaction and does not require precise hydrogen bonding between the substituted amino acid and the end of the alpha-helix.

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Year:  1988        PMID： 3200317     DOI： 10.1038/336651a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  53 in total

Review 1.  Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.

Authors:  C Vieille; G J Zeikus
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species.

Authors:  P J Haney; J H Badger; G L Buldak; C I Reich; C R Woese; G J Olsen
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

3.  Structure of a protein G helix variant suggests the importance of helix propensity and helix dipole interactions in protein design.

Authors:  P Strop; A M Marinescu; S L Mayo
Journal:  Protein Sci       Date:  2000-07       Impact factor: 6.725

4.  Long-distance conformational changes in a protein engineered by modulated sequence duplication.

Authors:  Martin Sagermann; Leslie Gay; Brian W Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-17       Impact factor: 11.205

5.  Dipoles localized at helix termini of proteins stabilize charges.

Authors:  J Aqvist; H Luecke; F A Quiocho; A Warshel
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

Review 6.  Enzyme stabilization: state of the art.

Authors:  L Gianfreda; M R Scarfi
Journal:  Mol Cell Biochem       Date:  1991-02-02       Impact factor: 3.396

Review 7.  Stability of protein pharmaceuticals.

Authors:  M C Manning; K Patel; R T Borchardt
Journal:  Pharm Res       Date:  1989-11       Impact factor: 4.200

8.  Position dependence of the 13C chemical shifts of alpha-helical model peptides. Fingerprint of the 20 naturally occurring amino acids.

Authors:  Jorge A Vila; Héctor A Baldoni; Harold A Scheraga
Journal:  Protein Sci       Date:  2004-11       Impact factor: 6.725

9.  A network representation of protein structures: implications for protein stability.

Authors:  K V Brinda; Saraswathi Vishveshwara
Journal:  Biophys J       Date:  2005-09-08       Impact factor: 4.033

10.  On the orientation of the backbone dipoles in native folds.

Authors:  Daniel R Ripoll; Jorge A Vila; Harold A Scheraga
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-13       Impact factor: 11.205
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