Sema Karakaş1, Cihan Kaya2, Hakan Güraslan2, Damlanur Sakiz3, Sema Süzen Çaypinar2, Hüseyin Cengiz3, Murat Ekin2, Levent Yaşar2. 1. Department of Obstetrics and Gynecology, University of Health Sciences,Gaziosmanpaşa Taksim Training and Research Hospital, İstanbul, Turkey 2. Department of Obstetrics and Gynecology, University of Health Sciences,Bakirköy Dr. Sadi Konuk Training and Research Hospital, İstanbul, Turkey 3. Department of Pathology, University of Health Sciences,Bakirköy Dr Sadi Konuk Training and Research Hospital, İstanbul, Turkey
Abstract
Background/aim: Adnexal torsion is a common gynaecological emergency, and considered to be a problem mostly in reproductive-age women. To evaluatethe effect of metformin and detorsion treatment on reducing ovarian reserve in an ovarian torsion model. Materials and methods: Twenty-four nonpregnant, Wistar Hannover rats were included in the study. Animals were divided into 3 groups: the control group, the detorsion only group, and the metformin + detorsion group. The first group received only laparotomy. In the second group, ovaries were fixed to the abdominal wall after performing 360° ovarian torsion, followed by detorsion after a 3-h period of ischemia. The third group underwent the same torsion and detorsion procedures as the second group, and received 50 mg/kg metformin by gavage for 14 days. Ovarian damage scores, follicle counts, and AMH levels were evaluated. Results: The total damage score was significantly increased in the detorsion only group compared to the metformin+detorsion and control groups. Pre-operative/post-operative AMH decreases were statistically significant in negative direction in the detorsion only group when compared to the metformin+detorsion and control groups (P = 0.001). Conclusion: Metformin+detorsion treatment may be effective in protecting the ovarian reserve after ovarian torsion. This work is licensed under a Creative Commons Attribution 4.0 International License.
Background/aim: Adnexal torsion is a common gynaecological emergency, and considered to be a problem mostly in reproductive-age women. To evaluatethe effect of metformin and detorsion treatment on reducing ovarian reserve in an ovarian torsion model. Materials and methods: Twenty-four nonpregnant, Wistar Hannover rats were included in the study. Animals were divided into 3 groups: the control group, the detorsion only group, and the metformin + detorsion group. The first group received only laparotomy. In the second group, ovaries were fixed to the abdominal wall after performing 360° ovarian torsion, followed by detorsion after a 3-h period of ischemia. The third group underwent the same torsion and detorsion procedures as the second group, and received 50 mg/kg metformin by gavage for 14 days. Ovarian damage scores, follicle counts, and AMH levels were evaluated. Results: The total damage score was significantly increased in the detorsion only group compared to the metformin+detorsion and control groups. Pre-operative/post-operative AMH decreases were statistically significant in negative direction in the detorsion only group when compared to the metformin+detorsion and control groups (P = 0.001). Conclusion:Metformin+detorsion treatment may be effective in protecting the ovarian reserve after ovarian torsion. This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors: Ali Ozler; Abdulkadir Turgut; Hatice Ender Soydinç; Muhammet Erdal Sak; Mehmet Siddik Evsen; Ulas Alabalik; Mustafa Kemal Basarali; Engin Deveci Journal: Reprod Sci Date: 2013-04-12 Impact factor: 3.060