Effect of bacillus bacterial extracts on the expression of major histocompatibility complex Class I and Class II receptors on MDA-MB 231 breast cancer cells.

OBJECTIVE: The expression of human leukocyte antigen (HLA)-ABC and HLA-DR is linked to the development of breast cancer. This study was aimed to determine the effect of bacillus bacterial extracts on the expression of the major histocompatibility complexes (MHC) Class I and Class II receptors on breast cancer cells.
METHODS: The expression of HLA-ABC and HLA-DR was assessed by flow cytometry and confocal microscope on the human breast tumor cell lines MDA-MB-231, which used a readily accessible model system. It is postulated that the HLA-ABC and HLA-DR receptors might be regulated by bacillus bacterial extracts designated (RA10, RA4, RA7, and RA16).
RESULTS: It was observed that the treatment of cell line MDA-MB-231 with the RA10 resulted in an upregulation of the cell surface expression of the HLA-A, B, C receptors. It was observed that RA10 has the capacity to reduce the expression of HLA-A, B, C, it also shows a detectable degree of cytotoxicity when used at high concentrations. The data show that the cell surface expression of HLA-ABC is higher than HLA-DR. No significant changes of HLA-DR expression were observed on MDA-MB-231 cell lines.
CONCLUSIONS: Improved understanding of the connection between HLA-ABC, HLA-DR, and bacterial extracts such as RA10 may lead to the development of drug design and therapies related to breast cancer condition in which these receptors are involved. Copyright: © International Journal of Health Sciences.