Zafar Rasheed1, Naila Rasheed2, Osama Al-Shaya3. 1. Department of Medical Biochemistry, College of Medicine, Qassim University, P.O. Box 6655, Buraidah, 51452, Saudi Arabia. zafarrasheed@qumed.edu.sa. 2. Department of Medical Biochemistry, College of Medicine, Qassim University, P.O. Box 6655, Buraidah, 51452, Saudi Arabia. 3. Department of Orthopedics, King Fahd Medical City, Riyadh, Saudi Arabia.
Abstract
PURPOSE: MicroRNAs (miRNAs) are short, non-coding RNAs involved in almost all cellular processes. Epigallocatechin-3-O-gallate (EGCG) is a green tea polyphenol and is known to exert anti-arthritic effects by inhibiting genes associated with osteoarthritis (OA). This study was undertaken to investigate the global effect of EGCG on interleukin-1β (IL-1β)-induced expression of miRNAs in human chondrocytes. METHODS: Human chondrocytes were derived from OA cartilage and then treated with EGCG and IL-1β. Human miRNA microarray technology was used to determine the expression profile of 1347 miRNAs. Microarray results were verified by taqman assays and transfection of chondrocytes with miRNA inhibitors. RESULTS: Out of 1347 miRNAs, EGCG up-regulated expression of 19 miRNAs and down-regulated expression of 17 miRNAs, whereas expression of 1311 miRNAs remains unchanged in IL-1β-stimulated human OA chondrocytes. Bioinformatics approach showed that 3`UTR of ADAMTS5 mRNA contains the 'seed-matched-sequence' for hsa-miR-140-3p. IL-1β-induced expression of ADAMTS5 correlated with down-regulation of hsa-miR-140-3p. Importantly, EGCG inhibited IL-1β-induced ADAMTS5 expression and up-regulated the expression of hsa-miR-140-3p. This EGCG-induced co-regulation between ADAMTS5 and hsa-miR-140-3p becomes reversed in OA chondrocytes transfected with anti-miR-140-3p. CONCLUSIONS: This study provides an important insight into the molecular basis of the reported anti-arthritic effects of EGCG. Our data indicate that the potential of EGCG in OA chondrocytes may be related to its ability to globally inhibit inflammatory response via modulation of miRNAs expressions.
PURPOSE: MicroRNAs (miRNAs) are short, non-coding RNAs involved in almost all cellular processes. Epigallocatechin-3-O-gallate (EGCG) is a green tea polyphenol and is known to exert anti-arthritic effects by inhibiting genes associated with osteoarthritis (OA). This study was undertaken to investigate the global effect of EGCG on interleukin-1β (IL-1β)-induced expression of miRNAs in human chondrocytes. METHODS:Human chondrocytes were derived from OA cartilage and then treated with EGCG and IL-1β. Human miRNA microarray technology was used to determine the expression profile of 1347 miRNAs. Microarray results were verified by taqman assays and transfection of chondrocytes with miRNA inhibitors. RESULTS: Out of 1347 miRNAs, EGCG up-regulated expression of 19 miRNAs and down-regulated expression of 17 miRNAs, whereas expression of 1311 miRNAs remains unchanged in IL-1β-stimulated human OA chondrocytes. Bioinformatics approach showed that 3`UTR of ADAMTS5 mRNA contains the 'seed-matched-sequence' for hsa-miR-140-3p. IL-1β-induced expression of ADAMTS5 correlated with down-regulation of hsa-miR-140-3p. Importantly, EGCG inhibited IL-1β-induced ADAMTS5 expression and up-regulated the expression of hsa-miR-140-3p. This EGCG-induced co-regulation between ADAMTS5 and hsa-miR-140-3p becomes reversed in OA chondrocytes transfected with anti-miR-140-3p. CONCLUSIONS: This study provides an important insight into the molecular basis of the reported anti-arthritic effects of EGCG. Our data indicate that the potential of EGCG in OA chondrocytes may be related to its ability to globally inhibit inflammatory response via modulation of miRNAs expressions.
Authors: T M Haqqi; D D Anthony; S Gupta; N Ahmad; M S Lee; G K Kumar; H Mukhtar Journal: Proc Natl Acad Sci U S A Date: 1999-04-13 Impact factor: 11.205
Authors: Salahuddin Ahmed; Ayesha Rahman; Absarul Hasnain; Matthew Lalonde; Victor M Goldberg; Tariq M Haqqi Journal: Free Radic Biol Med Date: 2002-10-15 Impact factor: 7.376
Authors: F Meng; Z Zhang; W Chen; G Huang; A He; C Hou; Y Long; Z Yang; Z Zhang; W Liao Journal: Osteoarthritis Cartilage Date: 2016-01-14 Impact factor: 6.576
Authors: Zafar Rasheed; Arivarasu N Anbazhagan; Nahid Akhtar; Sangeetha Ramamurthy; Frank R Voss; Tariq M Haqqi Journal: Arthritis Res Ther Date: 2009-05-15 Impact factor: 5.156
Authors: Ahmed A H Abdellatif; Zafar Rasheed; Ahmad H Alhowail; Abdulmajeed Alqasoumi; Mansour Alsharidah; Riaz A Khan; Abdullah S M Aljohani; Maha A Aldubayan; Waleed Faisal Journal: Int J Nanomedicine Date: 2020-10-30
Authors: Ahmed A H Abdellatif; Mansour Alsharidah; Osamah Al Rugaie; Hesham M Tawfeek; Nahla Sameh Tolba Journal: Drug Des Devel Ther Date: 2021-05-13 Impact factor: 4.162
Authors: Bruna J Quintanilha; Bruna Z Reis; Graziela B Silva Duarte; Silvia M F Cozzolino; Marcelo M Rogero Journal: Nutrients Date: 2017-10-27 Impact factor: 5.717