Literature DB >> 3198125

Isolation of human chromosome 21 sequences and their application to in situ hybridization.

K H Choo1, G Filby, E Earle, R Brown.   

Abstract

We report the isolation of 50 independent unique sequences from a human chromosome 21 library (identification code LA21 NSO1). These sequences were individually assigned to chromosome 21 using a mouse-human somatic hybrid cell line, WAVR 4d-F94a. Use of these unique clones as a mixture of probes for in situ hybridization of human metaphase chromosomes demonstrated strong signals on chromosome 21. These unique DNA sequences should provide useful tools for structural and functional analysis of human chromosome 21. The use of these sequences for the detection of Down syndrome is discussed.

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Year:  1988        PMID： 3198125     DOI： 10.1007/bf00283728

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  21 in total

1.  Presence of human chromosome 21 alone is sufficient for hybrid cell sensitivity to human interferon.

Authors:  D L Slate; L Shulman; J B Lawrence; M Revel; F H Ruddle
Journal:  J Virol       Date:  1978-01       Impact factor: 5.103

2.  Genomic organization of human centromeric alpha satellite DNA: characterization of a chromosome 17 alpha satellite sequence.

Authors:  K H Choo; R Brown; G Webb; I W Craig; R G Filby
Journal:  DNA       Date:  1987-08

3.  Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids.

Authors:  M L Van Keuren; P C Watkins; H A Drabkin; E W Jabs; J F Gusella; D Patterson
Journal:  Am J Hum Genet       Date:  1986-06       Impact factor: 11.025

4.  18p- syndrome: an unusual case and diagnosis by in situ hybridization with chromosome 18-specific alphoid DNA sequence.

Authors:  S G Vorsanova; Y B Yurov; I A Alexandrov; I A Demidova; S P Mitkevich; A F Tirskaia
Journal:  Hum Genet       Date:  1986-02       Impact factor: 4.132

5.  Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84.

Authors:  T Cremer; J Landegent; A Brückner; H P Scholl; M Schardin; H D Hager; P Devilee; P Pearson; M van der Ploeg
Journal:  Hum Genet       Date:  1986-12       Impact factor: 4.132

6.  Sequence heterogeneity within the human alphoid repetitive DNA family.

Authors:  P Devilee; P Slagboom; C J Cornelisse; P L Pearson
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

7.  Homologous alpha satellite sequences on human acrocentric chromosomes with selectivity for chromosomes 13, 14 and 21: implications for recombination between nonhomologues and Robertsonian translocations.

Authors:  K H Choo; B Vissel; R Brown; R G Filby; E Earle
Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

8.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

9.  Use of a chromosome 21 cloned DNA probe for the analysis of non-disjunction in Down syndrome.

Authors:  K E Davies; K Harper; D Bonthron; R Krumlauf; A Polkey; M E Pembrey; R Williamson
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

Review 10.  Molecular genetics of human chromosome 21.

Authors:  P C Watkins; R E Tanzi; S V Cheng; J F Gusella
Journal:  J Med Genet       Date:  1987-05       Impact factor: 6.318
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