Miguel Angel Aguilar-Luis1, Juana Del Valle-Mendoza2, Wilmer Silva-Caso3, Tamara Gil-Ramirez4, Saul Levy-Blitchtein5, Jorge Bazán-Mayra6, Victor Zavaleta-Gavidia7, Daniel Cornejo-Pacherres8, Carlos Palomares-Reyes9, Luis J Del Valle10. 1. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru. Electronic address: ma23aguilar@gmail.com. 2. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru. Electronic address: juana.delvalle@upc.pe. 3. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru. Electronic address: gian_will@hotmail.com. 4. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru. Electronic address: tamara.gil.1706@gmail.com. 5. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru. Electronic address: levysaul45@hotmail.com. 6. Laboratorio de Referencia, Dirección Regional de Salud de Cajamarca (DIRESA), Cajamarca, Peru. Electronic address: jbazanm@diresacajamarca.gob.pe. 7. Laboratorio de Referencia, Dirección Regional de Salud de Cajamarca (DIRESA), Cajamarca, Peru. Electronic address: vzavaletag@gmail.com. 8. Laboratorio de Referencia, Dirección Regional de Salud de Cajamarca (DIRESA), Cajamarca, Peru. Electronic address: dcornejop68@yahoo.es. 9. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru. Electronic address: carloscesarpalomaresreyes@gmail.com. 10. Barcelona Research Center for Multiscale Science and Engineering, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Barcelona, Spain. Electronic address: luis.javier.del.valle@upc.edu.
Abstract
BACKGROUND: The infection caused by Mayaro virus (MAYV), which presents as an acute febrile illness, is considered a neglected tropical disease. The virus is an endemic and emerging pathogen in South America and the Caribbean, responsible for occasional and poorly characterized outbreaks. Currently there is limited information about its expansion and risk areas. METHODS: A cross-sectional study was performed in 10 urban primary care health centers in the Cajamarca region of Peru from January to June 2017. A total of 359 patients with suspected febrile illness were assessed. RNA was extracted from serum samples, following which MAYV real-time reverse transcriptase PCR (RT-PCR) for the detection of the nsP1 gene was performed. RESULTS: MAYV was detected in 11.1% (40/359) of samples after RT-PCR amplification and confirmatory DNA sequencing. Most infections were detected in the adult population aged 18-39 years (40%) and 40-59 years (32.5%). Headache was the most frequent symptom in patients with MAYV infection (77.5%), followed by fever (72.5%), myalgia (55.0%), and arthralgia (50.0%). During the study, most of the MAYV cases were seen in May (47.5%) and April (35.0%), corresponding to the dry season (months without rain). CONCLUSIONS: This study is novel in describing the presence of MAYV in Cajamarca, an Andean region of Peru. Symptoms are non-specific and can be confused with those of other arbovirus or bacterial infections. Molecular biology methods such as RT-PCR allow the timely and accurate detection of MAYV and could thus be considered as a tool for surveillance in endemic areas.
BACKGROUND: The infection caused by Mayaro virus (MAYV), which presents as an acute febrile illness, is considered a neglected tropical disease. The virus is an endemic and emerging pathogen in South America and the Caribbean, responsible for occasional and poorly characterized outbreaks. Currently there is limited information about its expansion and risk areas. METHODS: A cross-sectional study was performed in 10 urban primary care health centers in the Cajamarca region of Peru from January to June 2017. A total of 359 patients with suspected febrile illness were assessed. RNA was extracted from serum samples, following which MAYV real-time reverse transcriptase PCR (RT-PCR) for the detection of the nsP1 gene was performed. RESULTS:MAYV was detected in 11.1% (40/359) of samples after RT-PCR amplification and confirmatory DNA sequencing. Most infections were detected in the adult population aged 18-39 years (40%) and 40-59 years (32.5%). Headache was the most frequent symptom in patients with MAYVinfection (77.5%), followed by fever (72.5%), myalgia (55.0%), and arthralgia (50.0%). During the study, most of the MAYV cases were seen in May (47.5%) and April (35.0%), corresponding to the dry season (months without rain). CONCLUSIONS: This study is novel in describing the presence of MAYV in Cajamarca, an Andean region of Peru. Symptoms are non-specific and can be confused with those of other arbovirus or bacterial infections. Molecular biology methods such as RT-PCR allow the timely and accurate detection of MAYV and could thus be considered as a tool for surveillance in endemic areas.
Authors: Lauren E Williamson; Kristen M Reeder; Kevin Bailey; Minh H Tran; Vicky Roy; Mallorie E Fouch; Nurgun Kose; Andrew Trivette; Rachel S Nargi; Emma S Winkler; Arthur S Kim; Christopher Gainza; Jessica Rodriguez; Erica Armstrong; Rachel E Sutton; Joseph Reidy; Robert H Carnahan; W Hayes McDonald; Clara T Schoeder; William B Klimstra; Edgar Davidson; Benjamin J Doranz; Galit Alter; Jens Meiler; Kevin L Schey; Justin G Julander; Michael S Diamond; James E Crowe Journal: Cell Date: 2021-08-19 Impact factor: 66.850
Authors: Miguel Angel Aguilar-Luis; Juana Del Valle-Mendoza; Isabel Sandoval; Wilmer Silva-Caso; Fernando Mazulis; Hugo Carrillo-Ng; Yordi Tarazona-Castro; Johanna Martins-Luna; Ronald Aquino-Ortega; Isaac Peña-Tuesta; Angela Cornejo-Tapia; Luis J Del Valle Journal: BMC Res Notes Date: 2021-01-21