Lei Zhou1, Jie-Hui Tan1, Wan-Yan Zhou2, Jia Xu3, Shi-Jing Ren4, Zhen-Yu Lin1, Xue-Mei Chen5, Guo-Wei Zhang6. 1. Division of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou, 510515, People's Republic of China. 2. Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China. 3. Department of Pathophysiology, Southern Medical University, Guangzhou, China. 4. Department of Endocrinology, Nanfang Hospital, Southern Medical University, Guangzhou, China. 5. Department of Occupational Health and Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China. 6. Division of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou, 510515, People's Republic of China. guoweizhang77@163.com.
Abstract
BACKGROUND AND AIMS: Acute pancreatitis (AP) is a severe pancreatic disorder that remains associated with high mortality due to a lack of effective drugs and management strategies. This study aimed to investigate the molecular pathogenic mechanisms of AP involving p53 and endoplasmic reticulum (ER) stress pathways. METHODS: Expression of PRSS1 and p53 in human AP tissues was detected by immunohistochemistry and Western blotting. AP was induced with caerulein in humanized PRSS1 transgenic mice, and its severity was verified by histological imaging, evaluation of edema, serum amylase, and trypsin activity assays. A transferase-mediated d-UTP nick end-labeling assay was performed to evaluate acinar cell apoptosis associated with AP. The expression of ER stress genes was assessed by quantitative RT-PCR (qRT-PCR) and Western blotting. RESULTS: PRSS1 and p53 were highly expressed in human AP tissues. Expression of human PRSS1 in caerulein-treated mice induced significant acinar cell apoptosis and AP progression. P53 knockout significantly suppressed AP progression in humanized PRSS1 transgenic mice. The ER stress pathway was activated by PRSS1 and mediated the progression of AP in mouse pancreatic tissues. Application of a p53 inhibitor effectively ameliorated caerulein-induced AP in PRSS1 transgenic mice, while a p53 activator promoted the progression of AP. CONCLUSION: P53, which was activated by the ER stress pathway, promoted the progression of AP in mice expressing PRSS1 by inducing acinar cell apoptosis.
BACKGROUND AND AIMS: Acute pancreatitis (AP) is a severe pancreatic disorder that remains associated with high mortality due to a lack of effective drugs and management strategies. This study aimed to investigate the molecular pathogenic mechanisms of AP involving p53 and endoplasmic reticulum (ER) stress pathways. METHODS: Expression of PRSS1 and p53 in human AP tissues was detected by immunohistochemistry and Western blotting. AP was induced with caerulein in humanized PRSS1transgenic mice, and its severity was verified by histological imaging, evaluation of edema, serum amylase, and trypsin activity assays. A transferase-mediated d-UTP nick end-labeling assay was performed to evaluate acinar cell apoptosis associated with AP. The expression of ER stress genes was assessed by quantitative RT-PCR (qRT-PCR) and Western blotting. RESULTS:PRSS1 and p53 were highly expressed in human AP tissues. Expression of humanPRSS1 in caerulein-treated mice induced significant acinar cell apoptosis and AP progression. P53 knockout significantly suppressed AP progression in humanized PRSS1transgenic mice. The ER stress pathway was activated by PRSS1 and mediated the progression of AP in mouse pancreatic tissues. Application of a p53 inhibitor effectively ameliorated caerulein-induced AP in PRSS1transgenic mice, while a p53 activator promoted the progression of AP. CONCLUSION:P53, which was activated by the ER stress pathway, promoted the progression of AP in mice expressing PRSS1 by inducing acinar cell apoptosis.
Entities:
Keywords:
Acute pancreatitis; Apoptosis; ER stress; P53; PRSS1
Authors: Zhao Zhirong; Han Li; He Yiqun; He Chunyang; Zhou Lichen; Tan Zhen; Wang Tao; Dai Ruiwu Journal: Apoptosis Date: 2022-05-25 Impact factor: 5.561
Authors: Amanda K Casey; Hillery F Gray; Suneeta Chimalapati; Genaro Hernandez; Andrew T Moehlman; Nathan Stewart; Hazel A Fields; Burak Gulen; Kelly A Servage; Karoliina Stefanius; Aubrie Blevins; Bret M Evers; Helmut Krämer; Kim Orth Journal: Proc Natl Acad Sci U S A Date: 2022-08-01 Impact factor: 12.779