Literature DB >> 31969761

Breastfeeding Duration as a Predictor of Childhood Lifestyle Habits, Overweight and Obesity in Second- and Third-Grade Schoolchildren in Croatia.

Maja Lang Morović1, Sanja Musić Milanović1.   

Abstract

Breastfeeding is related to better overall health in adult life and is one of the commonly described protective factors for childhood obesity. The purpose of this study was to describe the relationship between breastfeeding duration, childhood lifestyle habits, overweight and obesity in a cohort of Croatian second- and third-grade schoolchildren. Randomly selected second- and third-grade children aged 6-11 years (N=5662) were measured for weight and height in order to calculate nutritional status for each child. Also, a self-reported questionnaire was filled in by the parents to gather information on the child including breastfeeding duration, dietary and physical activity habits, and health risk behaviors. Regression analyses were performed to explore associations between breastfeeding duration and the odds of having dietary, physical activity or overall health risk behaviors, or of being overweight or obese. Significant associations were found showing that children who were breastfed for less than 6 months had higher odds for being overweight (ORadj=1.24; 95% CI 1.04-1.47) or obese (ORadj=1.25; 95% CI 1.02-1.53). After adjusting for confounders, breastfeeding for less than 6 months was not found to be predictive of dietary, physical activity or overall health risk behaviors. In conclusion, breastfeeding for longer than 6 months is a protective factor for overweight and obesity in 6-to 11-year-old children in Croatia, but not for healthy lifestyle development.

Entities:  

Keywords:  Breastfeeding; Child; Croatia; Health risk behaviors; Healthy lifestyle; Nutritional status; Obesity; Protective factors

Mesh:

Year:  2019        PMID: 31969761      PMCID: PMC6971800          DOI: 10.20471/acc.2019.58.03.12

Source DB:  PubMed          Journal:  Acta Clin Croat        ISSN: 0353-9466            Impact factor:   0.780


Introduction

With continuing progression of science in the area, public health professionals are gaining more insight into the relationships between health risk factors and mechanisms of development and prevention of non-communicable diseases. Consequently, it is becoming clear that programming of adult disease outcomes emerges early in life and that, in particular, the first 1000 days play a predominant role in lifelong health (). One of the most often-described protective factors for health is breastfeeding. Long-term, physical and psychological benefits of breastfeeding for both infant and mother are numerous and widely described (). The World Health Organization (WHO) recommends initiation of breastfeeding within the first hour after birth and continuation of exclusive breastfeeding until at least six months-of-age when complementary food should be gradually introduced. Nevertheless, it is advised to continue breastfeeding until the child is at least two years old (). Breast milk is the newborns’ first and best food, it is free, and is often referred to as the ‘individualized medicine’ (). However, the prevalence of breastfeeding remains rather low. In the United States in 2015, the mean prevalence of women exclusively breastfeeding their children by the age of three months was 46.9%, and by 6 months 24.9% (). The prevalence of breastfeeding in Europe is somewhat higher. In 2005, 59.2% of infants were breastfed at the age of 3 months and 47.3% of infants were breastfed at the age of 6 months (, ). Among systematically examined infants in Croatia in 2016, 78.8% of 0-2 month-old infants were exclusively breastfed, 10.9% were fed with breast milk and its substitutes, 10.3% were formula-fed, whereas for 25.4% data on infant feeding type were lacking. After the first three months of life, the proportion of infants exclusively breastfed decreased to 71.7%, and the proportion of infants exclusively breastfed after the sixth month of age decreased to 10.7% (). Besides its protective function in fighting infections in infancy, breastfeeding is related to better overall health in adult life, and is an unsubstitutable mechanism of prevention of non-communicable diseases (). Breastfeeding has also been found to be a protective factor for obesity (). Obesity represents a risk factor for today’s leading non-communicable diseases including cardiovascular diseases, type 2 diabetes, chronic obstructive pulmonary disease, some types of cancers and mental diseases (). Obese children often become obese adults, making the health risk even greater (). Over the last three decades, there has been a rise in childhood obesity worldwide, regardless of the level of development of the country (). The prevalence of childhood obesity is at its historically highest level, and it presents one of the major public health concerns and priorities worldwide, being called a time bomb for future demands on healthcare services (). The etiology of obesity in childhood is complex. It is a result of interplay between genetic, prenatal and environmental factors, early life determinants, and individual lifestyle habits (). Being the first food, breastfeeding practices in infancy are related to food choices and dietary habits later in life (). Also, mothers who breastfeed their children for a recommended period of time are setting building blocks of healthy lifestyle habits for their offspring. Along that note, the purpose of this study was to examine the protective power of breastfeeding lasting for six months or longer on childhood lifestyle habits observed as food and physical activity risk behaviors, overweight and obesity in a cohort of Croatian second- and third-grade schoolchildren.

Subjects and Methods

This study was conducted as part of the WHO Europe Childhood Obesity Surveillance Initiative (COSI), for Croatian purposes designed to trace obesity prevalence in 8.0-8.9 year-old-children (). COSI was established as a coordinated surveillance system among children in elementary school in order to gather Europe-wide, comparable data on children’s anthropometric measurements including body height, weight, and waist and hip circumference to record and monitor trends in nutritional status among children aged 6.0-9.9 years. The general design of the COSI research has been described in detail by the WHO team (). In Croatia, COSI was conducted in accordance with the COSI Protocol, initiated in the 2015/2016 academic year, during the fourth European round of study, and administered by the Croatian Institute of Public Health (CIPH) with support of the Ministry of Health (MoH), Ministry of Science, Education and Sports (MSES) and World Health Organization Regional Office for Europe. Sampling methods involved randomly selecting a nationally representative sample of second-grade classes. For every randomly selected second-grade class, a matching third-grade class from the same school was selected. Overall, 182 second-grade classes and 182 third-grade classes from 164 elementary schools were selected. All of the selected schools agreed to participate in the study. The overall response rate was 79.2% and the final sample included 5662 children aged 6 to 11 years (mean=8.6 years, SD=0.6). The information collecting conducted from October to December 2015 involved taking anthropometric measurements of children in the selected classes and was carried out in school facilities, mostly in gym halls or classrooms, during school-hours. Weight to the nearest 0.1 kg was measured using an electronic weight scale (SECA 877, Hamburg, Germany), while children were dressed in light gym clothing. Standing height was measured to the nearest 0.1 cm using stadiometer (SECA 217, Hamburg, Germany). For accuracy, the height was measured twice and the mean value of the two measurements was used on analysis. Body mass index (BMI) for age was calculated for each child as weight in kilograms divided by height in centimeters in order to obtain the WHO nutritional categories for children (). As part of the research, a self-reported questionnaire, the COSI Family Form, was completed by the parents to gather information on the child’s physical activity and dietary habits, as well as family lifestyle data including short family medical history and socioeconomic status. More detailed description of the study sample is presented in Table 1.
Table 1

Characteristics of study sample (N=5662)

Variable
Age (years), mean (SD)8.6 (0.6)
Birth weight (g), mean (SD)3428.1 (545.5)
Gender, n (%):  Girls  Boys2808 (49.6)2854 (50.4)
Breastfeeding, n (%):  Never breastfed  1-3 months of breastfeeding  4-6 months of breastfeeding  6-12 months of breastfeeding  12-24 months of breastfeeding  24+ months of breastfeeding  Missing data515 (9.4)1342 (23.7)1002 (17.7)1344 (23.7)887 (15.7)291 (5.1)281 (4.7)
Level of urbanization, n (%):  Urban  Semi-urban  Rural3245 (57.3)1714 (30.3)700 (12.4)
Maternal educational level, n (%):  Elementary school  High school  College degree or higher339 (7.2)2823 (60.3)1520 (32.5)
Risk behaviors, n (%):  Food riska,d  Low  Medium  High2471 (47.6)2430 (46.8)291 (5.6)
Physical activity riskb,d  Low  High4369 (88.9)543 (11.1)
Overall health riskc,d  Low  Medium  High1880 (40.5)2569 (55.3)197 (4.2)
Nutritional statuse, n (%):  Thinness  Normal weight  Overweight  Obese55 (1.0)3567 (63.0)1209 (21.3)831 (14.7)

SD = standard deviation; % is stated as valid percentage (excluding missing data), unless otherwise stated;

aFood risk behavior score ranged 0-8 and was derived from the Family Form regarding the child’s dietary habits. One point was assigned if the child was having breakfast <7 days/week, eating foods like potato chips (crisps), corn chips, popcorn or peanuts >3 days/week, eating foods like candy bars or chocolate >3 days/week, eating foods like biscuits, cakes, doughnuts or pies >3 days/week, and eating foods like pizza, French fries (chips), hamburgers, sausages or meat pies >3 days/week. Scores 0-2 presented low, 3-5 moderate and 6-8 high risk;

bPhysical activity risk behavior score ranged 0-5 and was derived from the Family Form regarding the child’s physical activity habits. One point was assigned if the child was using inactive transportation going to and from the school, going to a sports or dancing club <2 h/week, playing outside <1 h/day, spending time in front of the screen ≥2 h/day, sleeping <9 h/day. Scores 0-2 presented low and 3-5 high risk;

cHealth risk behavior score was created for each child by combining the ‘food-risk behavior score’ and the ‘physical activity-risk behavior score’ ranging from 0 (none of the health-risk behaviors present) to 13 points (all health-risk behaviors present). Scores 0-3 presented low, 4-6 moderate and 7-13 high risk;

dRisk scores used were based on previous research by Wijnhoven et al. ();

eNutritional status was defined according to the WHO criteria ().

SD = standard deviation; % is stated as valid percentage (excluding missing data), unless otherwise stated; aFood risk behavior score ranged 0-8 and was derived from the Family Form regarding the child’s dietary habits. One point was assigned if the child was having breakfast <7 days/week, eating foods like potato chips (crisps), corn chips, popcorn or peanuts >3 days/week, eating foods like candy bars or chocolate >3 days/week, eating foods like biscuits, cakes, doughnuts or pies >3 days/week, and eating foods like pizza, French fries (chips), hamburgers, sausages or meat pies >3 days/week. Scores 0-2 presented low, 3-5 moderate and 6-8 high risk; bPhysical activity risk behavior score ranged 0-5 and was derived from the Family Form regarding the child’s physical activity habits. One point was assigned if the child was using inactive transportation going to and from the school, going to a sports or dancing club <2 h/week, playing outside <1 h/day, spending time in front of the screen ≥2 h/day, sleeping <9 h/day. Scores 0-2 presented low and 3-5 high risk; cHealth risk behavior score was created for each child by combining the ‘food-risk behavior score’ and the ‘physical activity-risk behavior score’ ranging from 0 (none of the health-risk behaviors present) to 13 points (all health-risk behaviors present). Scores 0-3 presented low, 4-6 moderate and 7-13 high risk; dRisk scores used were based on previous research by Wijnhoven et al. (); eNutritional status was defined according to the WHO criteria ().

Study variables

Data on the duration of breastfeeding were obtained from the COSI Family Forms. Parents or other caretakers were asked if the child had ever been breastfed and for how long, but not whether the child had been exclusively breastfed. Breastfeeding was observed as a dichotomous predictor of health risks in children. Children who had never been breastfed were excluded from further analysis, and the breastfed children were grouped into two categories of children breastfed for less than 6 months and children breastfed for 6 months or longer in accordance with the WHO recommendations (). Health risk variables included child’s lifestyle habits and child’s weight status. Child’s lifestyle habits were observed as three variables, i.e. ‘food risk’, ‘physical activity risk’ and ‘health risk’. These risk variables were previously used by Wijnhoven et al. (). The food risk variable was created for each child as a score ranging from 0 (no risk) to 8 (maximum risk), based on the presence of eight food risk behaviors. Food risk behaviors were the following: having breakfast <7 days/week, eating fruit <7 days/week, eating vegetables (excluding potatoes) <7 days/week, drinking soft drinks containing sugar >3 days/week, eating foods like candy bars or chocolate >3 days/week, eating foods like biscuits, cakes, doughnuts or pies >3 days/week, eating foods like potato chips, corn chips, popcorn or peanuts >3 days/week and eating foods like pizza, French fries (chips), hamburgers, sausages or meat pies >3 days/week. Based on the scores, the categorical food risk variable was created. Scores 0-2 were referred to as low food risk, 3-5 as moderate, and 6-8 as high food risk. Physical activity risk score was also calculated as a sum of scores for each child. Each physical activity risk behavior was attributed one point. Physical activity risk was calculated based on the following behaviors: using inactive transportation going to and from the school, going to a sports or dancing club <2 h/week, actively playing outside <1 h/day, spending screen time ≥2 h/day, and sleep duration <9 h/day. The score range was 0-5 and was divided into two categories, i.e. 0-2 considered as low and 3-5 as high physical activity risk. The shares of children who had risk behaviors for each food risk variable and each physical activity risk variable used to calculate the score are shown in Table 2.
Table 2

Prevalence of individual food and physical activity risk behaviors (N=5662)

Variable
Food risk, n (%):
  Having breakfast <7 days/week1316 (24.1)
  Eating fruit <7 days/week3629 (66.2)
  Eating vegetables (excluding potatoes) <7 days/week4488 (82.7)
  Drinking soft drinks containing sugar >3 days/week1639 (30.4)
  Eating foods like candy bars or chocolate >3 days/week1718 (31.4)
  Eating foods like biscuits, cakes, doughnuts or pies >3 days/week1470 (26.8)
  Eating foods like pizza, French fries (chips), hamburgers, sausages or meat pies >3 days/week276 (5.0)
  Eating foods like potato chips (crisps), corn chips, popcorn or peanuts >3 days/week655 (12.0)
Physical activity risk, n (%):
  Using inactive transportation going to and from the school1691 (30.7)
  Going to a sports or dancing club <2 h/week1930 (35.2)
  Actively playing outside <1 h/day458 (5.6)
  Spending screen time ≥2 h/day2746 (52.4)
  Sleep duration <9 h/day332 (6.1)

% is stated as valid percentage (excluding missing data)

% is stated as valid percentage (excluding missing data) As a sum of food and physical activity risk scores, another variable of the health risk score was created. The health risk score ranged from 0 (no risk behaviors present) to 13 (all risk behaviors present) and was divided into the following three risk categories: no risk (0-3 points), moderate risk (4-7 points) and high risk (8-13 points). Food, physical activity and health risk scores were attributed to children who had no missing values (n=4646). Weight status variables included childhood ‘overweight’ and ‘obesity’ variables derived from child’s BMI and based on the WHO criteria (). Confounding variables in the study were age in months, sex, birth weight in grams, level of urbanization of the child’s place of living (in accordance with the Croatian Bureau of Statistics definition of urban, semi-urban and rural settings ()), and finally, socioeconomic characteristics of the family, described by the highest finished level of maternal formal education. All investigated variables were acquired from the COSI Family Form that involved self-reporting by parents or other caretakers.

Ethics

The Ethics Committee of the Croatian Institute of Public Health approved the study in July 2015 (Class: 602-01/15-01/0242). The research was instigated following the ethical principles of autonomy, beneficence and nonmaleficence, and was conducted in accordance with the WHO COSI Protocol (). All procedures were in accordance with ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008.

Statistics

All statistical analyses were performed using the SPSS Statistics 21.0 statistical package (IBM Corporation, Chicago, IL, USA). All analyses were performed on a weighted sample to account for nonresponse and noncoverage. Proportions were computed for categorical variables. The relationships between breastfeeding duration as a dichotomous variable and other variables were analyzed by χ2-test. Further, simple ordinal logistic regression analyses adjusted for child’s age and sex were performed, and odds ratios (OR) and 95% confidence intervals (CI) were obtained to explore associations between breastfeeding duration for more or less than 6 months and the odds of having overall health risk behaviors. The test of parallel lines was carried out to test the proportional odds assumption and the assumption was not rejected. For the association between food risk and breastfeeding duration, multinomial logistic regression was used because of non-proportional odds in the adjusted model. The association between breastfeeding duration and physical activity risk was tested using binary logistic regression. Multinomial logistic regression was also used to test the association between breastfeeding duration and nutritional status. Finally, multivariable logistic regression analyses, adjusted for socio-demographic factors including age, sex, birth weight in grams, urbanization level, parental education, and risk factors including nutritional status, food and physical risk were performed and OR and 95% CI were calculated to further analyze associations between breastfeeding duration for more or less than 6 months and the odds of having food, physical activity or overall health risk or being overweight or obese. Results were analyzed at α=0.05.

Results

The initial sample included 5662 children who were present at the school on the day of measurements. The study subgroup included 4866 children who had been breastfed. The subgroup included in regression analyses consisted of 3383 subjects that had no missing data on any of the variables in the model. The proportions of children breastfed for less than 6 months and for 6 months or longer, according to study variables, are presented in Table 3. These results confirmed significant differences between the children breastfed for less than 6 months and for 6 months or longer in relation to urbanization level, maternal education level, food risk, overall health risk and nutritional status. The children living in urban areas tended to be less frequently breastfed for less than six months (34.4%) than the others. The same held for the children whose mothers had a college degree or higher (28.6%), children with low food risk (34.6%) and low overall health risk (33.7%), and children with normal weight (35.5%).
Table 3

Proportions of children breastfed for 6 months or longer and for less than 6 months according to gender, urbanization, maternal education, weight status, and food, physical activity and overall health risks

Breastfeeding <6 months(n=1817)Breastfeeding ≥6 months(n=3049)
%n%nχ2df
Gender:
  Girls37.189362.915160.151
  Boys37.692462.41533
Urbanization level:
  Urban34.495465.6182224.182**
  Semi-urban41.261258.8873
  Rural41.424958.6353
Maternal education:
  Elementary school38.611461.418177.252**
  High school42.9107157.11426
  College degree or higher28.639671.4988
Food riska:
  Low34.676665.4144812.922*
  Medium39.583560.51281
  High41.310758.7152
Physical activity riskb:
  Low37.2144362.824330.6351
  High39.118060.9280
Overall health riskc:
  Low34.658565.411069.5702*
  Medium39.488360.61358
  High38.26861.8110
Nutritional statusd:
  Underweight37.51562.52513.7893*
  Physiological weight35.5109564.51990
  Overweight39.541060.5628
  Obesity42.329357.7400

df = degree of freedom; *p<0.01; **p<0.001

df = degree of freedom; *p<0.01; **p<0.001 Table 4 presents associations between breastfeeding for less than 6 months and study variables adjusted for age and sex. Significant associations were found showing that children who were breastfed for less than 6 months had higher odds for medium food risk (OR=1.24, 95% CI 1.07-1.43), higher odds for overall health risk (OR=1.19, 95% CI 1.04-1.37), and higher odds for being overweight (OR=1.21, 95% CI 1.02-1.43) or obese (OR=1.26, 95% CI 1.04-1.54). We found no significant associations between breastfeeding shorter than 6 months and physical activity and high food risk behaviors in schoolchildren.
Table 4

Simple and adjusted associations between childhood risk factors, overweight and obesity and breastfeeding duration <6 months in the group of children aged 6-11 years in Croatia (n=3383)

OR (CI)ORadj (CI)
Food riska:  Medium  High1.24* (1.07-1.43)1.28 (0.93-1.76)1.16 (1.00-1.34)1.15 (0.83-1.59)
Physical activity high riskb1.06 (0.84-1.33)0.96 (0.76-1.22)
Overall health riskc1.19* (1.04-1.37)1.07 (0.93-1.23)
Nutritional statusd  Overweight  Obesity1.21*(1.02-1.43)1.26*(1.04-1.54)1.24*(1.04-1.47)1.25*(1.02-1.53)

OR = odds ratio, CI = confidence interval;

aFood risk, an ordinal variable; odds ratios were calculated using multinomial logistic regression because of non-proportional odds in the adjusted model; low risk was set as a reference value;

bPhysical activity risk, a dichotomous variable; odds ratios were calculated using binary logistic regression, low risk was set as a reference value;

cHealth risk, an ordinal variable; odds ratios were calculated using ordinal regression; the proportional odds assumption was tested with the test of parallel lines and the assumption held both in the simple model (p=0.159) and adjusted model (p=0.240);

dNutritional status defined according to the WHO criteria (); odds ratios were calculated using multinomial logistic regression with normal weight set as a reference value.

OR = odds ratio, CI = confidence interval; aFood risk, an ordinal variable; odds ratios were calculated using multinomial logistic regression because of non-proportional odds in the adjusted model; low risk was set as a reference value; bPhysical activity risk, a dichotomous variable; odds ratios were calculated using binary logistic regression, low risk was set as a reference value; cHealth risk, an ordinal variable; odds ratios were calculated using ordinal regression; the proportional odds assumption was tested with the test of parallel lines and the assumption held both in the simple model (p=0.159) and adjusted model (p=0.240); dNutritional status defined according to the WHO criteria (); odds ratios were calculated using multinomial logistic regression with normal weight set as a reference value. The associations were further analyzed in multivariate regressions, presented in Table 4, adjusted for age, gender, birth weight, urbanization level, maternal education level and other risk factors. The only association that remained significant was the one between breastfeeding duration and nutritional status. Even when adjusted for other factors, children who were breastfed for less than 6 months had higher odds for being overweight (ORadj=1.24, 95% CI 1.04-1.47) or obese (ORadj=1.25, 95% CI 1.02-1.53). These results indicated that breastfeeding duration could be considered as one of important predictors of nutritional status in school age children. Simple models were adjusted for gender and age. The reference category for breastfeeding was ‘duration for at least 6 months’. The sample included only those cases who had no missing data on all the variables used in the model. Children who were categorized as thinness according to the WHO criteria were excluded from analysis. Models were adjusted for socio-demographic factors including gender, age, birth weight, parental education, urbanization level and risk factors including overweight, obesity, food and physical activity. Models were not adjusted for health risk as the sum of food and physical activity risks.

Discussion

We examined the association between breastfeeding duration and lifestyle habits, overweight and obesity in second- and third-grade schoolchildren in Croatia. Significant associations were found showing that children who were breastfed for less than 6 months had higher odds for being overweight and obese, even when adjusted for a variety of confounders such as child’s age, sex and birth weight, urbanization level, maternal education level and child’s other risk behaviors. Without adjusting for other factors, children who were breastfed for 6 months and longer tended to have healthier dietary habits, i.e. eating breakfast every day, eating more fruit and vegetable and less foods high in sugars, salt and trans-fatty acids. Previous studies have confirmed that breastfeeding duration is associated with healthier food choices in children (). This association can be observed from early childhood and can be explained by the fact that children’s food preferences appear to be shaped by exposure to foods and flavors from mother’s diet in the pre- and postnatal period () via amniotic fluid and breast milk (). For this reason, at the time of the introduction of solid foods, breastfed infants will have had more experience with tastes in comparison to their non-breastfed peers, which makes it easier for them to accept newly introduced flavors (). Breastfeeding therefore provides a solid foundation for development of healthy eating habits in children. When adjusted for other socio-demographic factors (maternal education and urbanization), physical activity risk behaviors, birth weight and current nutritional status, the association between breastfeeding and food risk was no longer significant. This indicated that breastfeeding duration could not be observed as a predictor of healthy dietary habits by itself. Children’s dietary habits are greatly influenced by mother’s and family’s characteristics. These influences begin even before the introduction of solid foods () and mothers who choose to breastfeed are taking an important first step on the road to developing healthy dietary habits in the child. The next step is the weaning period during which complementary foods are introduced and lifelong eating habits may be established. It is therefore recommended to adhere to dietary guidelines during this period in order to develop healthy eating habits in children and prevent development of risk behaviors, and consequently, overweight and obesity in the child (, ). The same pattern appears with the association of duration of breastfeeding under 6 months and overall health risk, which combines food and physical activity risk factors. These two risk behaviors combined are recognized to increase the risk of obesity and, consequently, development of a number of associated chronic conditions (). Although physical inactivity and screen-time as a sole risk factor was not found to be significantly associated with the duration of breastfeeding, breastfeeding for less than 6 months was a significant predictor of overall health risk when food and physical activity were combined. This result could be explained by the fact that food items contribute to the overall score more than physical activity items. Again, when adjusted for confounding factors, there was no significant association. In both adjusted and unadjusted models we found a significant association showing that children who were breastfed for less than 6 months had higher odds for both overweight and obesity in school age than children who were breastfed for 6 months and longer. These results are in line with previous research indicating that breastfed children had lower odds of being overweight (), and once again confirm the protective power of breastfeeding in childhood overweight and obesity development. Although several studies dispute this relationship (), most of them show positive effects of breastfeeding on childhood overweight and obesity development. This positive effect of breastfeeding on child’s weight has been hypothesized to be related to several biological mechanisms such as the role of breastfeeding in the regulation of infant appetite. Breastfed infants in comparison to formula-fed infants tend to have better control of their appetite, which enables them to be more proficient in hunger detection and limit food intake, a trait that persists to adulthood. Furthermore, breast milk contains leptin, which also may influence leptin level in childhood and later on in adolescence and adult life, but also helps regulate appetite and calorie metabolism in breastfed children (). Lastly, breastfeeding seems to be related to lower protein ingestion in comparison to formula feeding and this may lead to lipogenesis, development of fat cells and, without doubt, obesity (). Our study had several limitations. First, there was a problem of recall bias since we used data on breastfeeding that were collected several years later. Second, considering the participation rate (79.2%) and exclusion due to partial missing data, there was a certain selection bias. The sample that was selected for regression analysis was significantly different from the unselected one in the distribution of weight status, food risk, physical risk and overall health risk scores. The selected sample was more frequently overweight or obese but less frequently had high food, physical and overall health risk scores. Third, as noted, we only had data on the general duration of breastfeeding, and exclusivity of breastfeeding was not asked. The insight into exclusivity of breastfeeding would give us more detailed information on the benefits of breastfeeding for study outcomes. Fourth, since this was an observational study not designed to assess associations between breastfeeding and health risk behaviors or overweight/obesity, we had no information on the children’s first foods and physical activity habits in early childhood, which would be beneficial in explaining the role of family and parenting styles in the child’s habitual development. Another study, similar to ours, but with longitudinal character is therefore recommended. Fifth, we could only infer the implication of parenting styles and family functioning as these traits were not measured. Having these data could yield different results. Despite these limitations, this was the first study to show the protective power of breastfeeding for longer than 6 months on childhood overweight and obesity status in Croatia. The results also indicated that breastfeeding duration could not be used as a predictor of healthy lifestyle habits. It is important to point out once again that we did not test breastfeeding as a predictor of health-related outcomes but only the duration of breastfeeding in case that the child was breastfed at all. The findings from this study, drawn from a large, nationally representative random sample of children can be used in modeling and implementation of future public health and health promotion policies in promoting breastfeeding for longer than 6 months as a first step towards obesity prevention in children. These findings are also valuable in clinical practice and community nurse child visits. Pediatricians and community nurses should consider benefits of breastfeeding for longer than 6 months as the foundation of infant feeding and its role in child growth and development when counseling families who plan pregnancy, childbearing families and families with newborns and infants. It would also be helpful for providers to support and advise parents on the importance of complementary feeding, healthy eating guidelines and physical activity in early childhood, and suggest ways how parents can be active with their child. Establishing healthy eating and physical activity habits early in life could prove beneficial in halting the rise of childhood obesity worldwide.
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Authors:  Katherine I Disantis; Bradley N Collins; Jennifer O Fisher; Adam Davey
Journal:  Int J Behav Nutr Phys Act       Date:  2011-08-17       Impact factor: 6.457

Review 9.  Systematic review of randomised controlled trials of interventions that aim to reduce the risk, either directly or indirectly, of overweight and obesity in infancy and early childhood.

Authors:  Sarah A Redsell; Barrie Edmonds; Judy Anne Swift; Aloysius Niroshan Siriwardena; Stephen Weng; Dilip Nathan; Cris Glazebrook
Journal:  Matern Child Nutr       Date:  2015-04-20       Impact factor: 3.092

10.  Food variety at 2 years of age is related to duration of breastfeeding.

Authors:  Jane A Scott; Tsz Ying Chih; Wendy H Oddy
Journal:  Nutrients       Date:  2012-10-15       Impact factor: 5.717

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Authors:  Halima S Twabi; Samuel O M Manda; Dylan S Small
Journal:  Matern Child Health J       Date:  2022-08-10

2.  Exclusive Breastfeeding for at Least Four Months Is Associated with a Lower Prevalence of Overweight and Obesity in Mothers and Their Children after 2-5 Years from Delivery.

Authors:  Maria Mantzorou; Dimitrios Papandreou; Georgios K Vasios; Eleni Pavlidou; Georgios Antasouras; Evmorfia Psara; Zainab Taha; Efthymios Poulios; Constantinos Giaginis
Journal:  Nutrients       Date:  2022-08-31       Impact factor: 6.706

3.  Breastfeeding and overweight/obesity among children and adolescents: a cross-sectional study.

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Journal:  BMC Pediatr       Date:  2022-06-16       Impact factor: 2.567

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