OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem cells (hUCMSCs) on the polarization of lipopolysaccharide-stimulated RAW264.7 macrophages. METHODS: Lipopolysaccharide-stimulated RAW264.7 macrophages were co-cultured with hUCMSCs in a Transwell system for 4 d, and then labelled with anti-F4/80, anti-CD86, and anti-CD206 antibodies for flow cytometry. The co-cultured supernatants were detected by enzyme-linked immunosorbent assay for prostaglandin E2. The co-cultured RAW264.7 macrophages were also lysed to measure the intracellular level of inducible nitric oxide synthase. RESULTS: There were significantly more F4/80+CD86+CD206+ RAW264.7 macrophages in the hUCMSCs-treated groups than the control group (P<0.001). The secretion of prostaglandin E2 by lipopolysaccharide-stimulated RAW264.7 macrophages was significantly inhibited in a dose-dependent manner with the addition of hUCMSCs (P<0.001). The expression of iNOS, the intracellular marker of M1 cells, was also significantly inhibited by hUCMSCs (P<0.05). CONCLUSION: hUCMSCs significantly polarize the lipopolysaccharide-stimulated RAW264.7 macrophages from a pro-inflammatory M1 subpopulation to an intermediate subpopulation of anti-inflammatory M2 macrophages, which are associated with a gradual decrease of iNOS and PGE2 levels. IJCEP
OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem cells (hUCMSCs) on the polarization of lipopolysaccharide-stimulated RAW264.7 macrophages. METHODS: Lipopolysaccharide-stimulated RAW264.7 macrophages were co-cultured with hUCMSCs in a Transwell system for 4 d, and then labelled with anti-F4/80, anti-CD86, and anti-CD206 antibodies for flow cytometry. The co-cultured supernatants were detected by enzyme-linked immunosorbent assay for prostaglandin E2. The co-cultured RAW264.7 macrophages were also lysed to measure the intracellular level of inducible nitric oxide synthase. RESULTS: There were significantly more F4/80+CD86+CD206+ RAW264.7 macrophages in the hUCMSCs-treated groups than the control group (P<0.001). The secretion of prostaglandin E2 by lipopolysaccharide-stimulated RAW264.7 macrophages was significantly inhibited in a dose-dependent manner with the addition of hUCMSCs (P<0.001). The expression of iNOS, the intracellular marker of M1 cells, was also significantly inhibited by hUCMSCs (P<0.05). CONCLUSION: hUCMSCs significantly polarize the lipopolysaccharide-stimulated RAW264.7 macrophages from a pro-inflammatory M1 subpopulation to an intermediate subpopulation of anti-inflammatory M2 macrophages, which are associated with a gradual decrease of iNOS and PGE2 levels. IJCEP
Authors: Bing Luan; Young-Sil Yoon; John Le Lay; Klaus H Kaestner; Susan Hedrick; Marc Montminy Journal: Proc Natl Acad Sci U S A Date: 2015-12-07 Impact factor: 11.205
Authors: Peter J Murray; Judith E Allen; Subhra K Biswas; Edward A Fisher; Derek W Gilroy; Sergij Goerdt; Siamon Gordon; John A Hamilton; Lionel B Ivashkiv; Toby Lawrence; Massimo Locati; Alberto Mantovani; Fernando O Martinez; Jean-Louis Mege; David M Mosser; Gioacchino Natoli; Jeroen P Saeij; Joachim L Schultze; Kari Ann Shirey; Antonio Sica; Jill Suttles; Irina Udalova; Jo A van Ginderachter; Stefanie N Vogel; Thomas A Wynn Journal: Immunity Date: 2014-07-17 Impact factor: 31.745