| Literature DB >> 31935680 |
Wenjing Wang1, Fengzhen Zhou1, Xiyao Cheng1, Zhengding Su1, Huiling Guo2.
Abstract
To effective capture and universal enrichment of His-tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate Fe3O4 nanoparticles, connect NTA, and Ni2+ to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His-tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of Fe3O4/MPS@PAA/NTA-Ni2+ was 93.4 mg/g. Compared with High-Affinity Ni-NTA Resin and Ni-NTA Magnetic Agarose Beads, Fe3O4/MPS@PAA/NTA-Ni2+ nanocomposites exhibited higher separation efficiency and binding capacity towards His-tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields.Entities:
Mesh：
Substances：
Year: 2020 PMID： 31935680 PMCID： PMC8676476 DOI： 10.1049/iet-nbt.2019.0271
Source DB: PubMed Journal: IET Nanobiotechnol ISSN： 1751-8741 Impact factor: 1.847