Zainab Al-Lawati1, Faryal A Khamis2, Aisha Al-Hamdani3, Moza Al-Kalbani4, Fatma Ali Ramadhan5, Thuria Rashid Al-Rawahi6, Muhannad F Al-Kobaisi7. 1. Department of Microbiology & Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman. 2. Department of Medicine, Royal Hospital, Oman. 3. Department of Pathology, Sultan Qaboos University Hospital, Oman. 4. Department of Obstructive and Gynecology, Sultan Qaboos University Hospital, Oman. 5. Department of Pathology, Royal Hospital, Oman. 6. Department of Obstructive and Gynecology, Royal Hospital, Oman. 7. Department of Microbiology & Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman. Electronic address: kobaisi@squ.edu.om.
Abstract
BACKGROUND: Persistent infection with high-risk (HR) HPV genotypes has been associated with cervical cancer, the third cancer affecting women in Oman with a crude incidence rate of 4.7 and mortality rate of 2.5 respectively. Other types of lower-risk (LR) HPV are associated with warts in both genders worldwide. OBJECTIVES: To assess the prevalence and genotype distribution of HPV and the risk factors among women with normal and abnormal cytology. METHODS: A cross sectional study conducted between September 2014 and April 2015. 258 cervical samples were obtained from women aged 18-68 years attending the Gynaecology Out-patient Clinic. HPV genotyping was performed using a multiplex real time-polymerase chain reaction (RT-PCR) assay. RESULTS: 22 different HPV genotypes were detected in 46 women (17.8%) and included 15 HR and 7 LR genotypes. Human immunodeficiency virus (HIV) patients (P = 0.052) and oral contraceptives users (P = 0.016) showed significant association with HPV infection. CONCLUSION: The most frequently observed HPV types were HR HPV 82 and LR HPV 54. These findings show that the predominant HPV genotypes in Oman are different from those seen in worldwide studies. This finding is important to determine the potential impact of preventive measures especially new vaccines to reduce the burden of cervical cancer.
BACKGROUND: Persistent infection with high-risk (HR) HPV genotypes has been associated with cervical cancer, the third cancer affecting women in Oman with a crude incidence rate of 4.7 and mortality rate of 2.5 respectively. Other types of lower-risk (LR) HPV are associated with warts in both genders worldwide. OBJECTIVES: To assess the prevalence and genotype distribution of HPV and the risk factors among women with normal and abnormal cytology. METHODS: A cross sectional study conducted between September 2014 and April 2015. 258 cervical samples were obtained from women aged 18-68 years attending the Gynaecology Out-patient Clinic. HPV genotyping was performed using a multiplex real time-polymerase chain reaction (RT-PCR) assay. RESULTS: 22 different HPV genotypes were detected in 46 women (17.8%) and included 15 HR and 7 LR genotypes. Human immunodeficiency virus (HIV) patients (P = 0.052) and oral contraceptives users (P = 0.016) showed significant association with HPV infection. CONCLUSION: The most frequently observed HPV types were HR HPV 82 and LR HPV 54. These findings show that the predominant HPV genotypes in Oman are different from those seen in worldwide studies. This finding is important to determine the potential impact of preventive measures especially new vaccines to reduce the burden of cervical cancer.
Authors: Catia Sias; Valerio Guarrasi; Claudia Minosse; Daniele Lapa; Franca Del Nonno; Maria Rosaria Capobianchi; Anna Rosa Garbuglia; Paola Del Porto; Paola Paci Journal: Front Microbiol Date: 2020-11-25 Impact factor: 5.640
Authors: Jude Ogechukwu Okoye; Chiemeka Franklin Chukwukelu; Simon Imakwu Okekpa; Samuel Ifedioramma Ogenyi; Ifeoma Nora Onyekachi-Umah; Anthony Ajuluchukwu Ngokere Journal: Asian Pac J Cancer Prev Date: 2021-09-01