Karim Nagi1,2, Ishita Gupta3,4,5, Nawaf Jurdi6, Ayesha Jabeen3,4,5, Amber Yasmeen7, Gerald Batist7,8, Semir Vranic3,4, Ala-Eddin Al-Moustafa9,10,11. 1. College of Medicine, QU Health, Qatar University, PO Box 2713, Doha, Qatar. knagi@qu.edu.qa. 2. Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, PO Box 2713, Doha, Qatar. knagi@qu.edu.qa. 3. College of Medicine, QU Health, Qatar University, PO Box 2713, Doha, Qatar. 4. Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, PO Box 2713, Doha, Qatar. 5. Biomedical Research Centre, QU Health, Qatar University, PO Box 2713, Doha, Qatar. 6. Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon. 7. Segal Cancer Centre, Lady Davis Institute for Medical Research/JGH, McGill University, Montreal, QC, H3A 0G4, Canada. 8. Oncology Department, McGill University, Montreal, QC, H3A 0G4, Canada. 9. College of Medicine, QU Health, Qatar University, PO Box 2713, Doha, Qatar. aalmoustafa@qu.edu.qa. 10. Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, PO Box 2713, Doha, Qatar. aalmoustafa@qu.edu.qa. 11. Biomedical Research Centre, QU Health, Qatar University, PO Box 2713, Doha, Qatar. aalmoustafa@qu.edu.qa.
Abstract
BACKGROUND: High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein-Barr virus (EBV) to initiate and/or enhance the progression of several types of human carcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in human breast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women. METHODS: A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study. RESULTS: We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile, we report that high-risk HPVs and EBV are co-present in 30/102 (29%) of the samples; more significantly, our results indicate that their co-presence is associated with tumor grade (p = 0.03). CONCLUSION: Our data revealed that HPVs and EBV are present/co-present in human breast cancer where they may play an important role in its development and/or progression; thus, we believe that further investigations are essential to confirm and elucidate the presence/co-presence of these oncoviruses and the underlying mechanisms of their interaction in breast carcinogenesis.
BACKGROUND: High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein-Barr virus (EBV) to initiate and/or enhance the progression of several types of humancarcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in humanbreast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women. METHODS: A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study. RESULTS: We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile, we report that high-risk HPVs and EBV are co-present in 30/102 (29%) of the samples; more significantly, our results indicate that their co-presence is associated with tumor grade (p = 0.03). CONCLUSION: Our data revealed that HPVs and EBV are present/co-present in humanbreast cancer where they may play an important role in its development and/or progression; thus, we believe that further investigations are essential to confirm and elucidate the presence/co-presence of these oncoviruses and the underlying mechanisms of their interaction in breast carcinogenesis.
Entities:
Keywords:
Breast cancer; EBV; HPV; Lebanese population; Tumor grade
Authors: Najla A Lakkis; Salim M Adib; Mona H Osman; Umayya M Musharafieh; Ghassan N Hamadeh Journal: Cancer Epidemiol Date: 2010-04-22 Impact factor: 2.984
Authors: Michel P Coleman; Manuela Quaresma; Franco Berrino; Jean-Michel Lutz; Roberta De Angelis; Riccardo Capocaccia; Paolo Baili; Bernard Rachet; Gemma Gatta; Timo Hakulinen; Andrea Micheli; Milena Sant; Hannah K Weir; J Mark Elwood; Hideaki Tsukuma; Sergio Koifman; Gulnar Azevedo E Silva; Silvia Francisci; Mariano Santaquilani; Arduino Verdecchia; Hans H Storm; John L Young Journal: Lancet Oncol Date: 2008-07-17 Impact factor: 41.316