Literature DB >> 3190654

Increased calcium sensitivity of chemically skinned human atria by myosin light chain kinase.

I Morano1, C Bächle-Stolz, A Katus, J C Rüegg.   

Abstract

We investigated the influence of myosin P-LC phosphorylation catalysed by calcium/calmodulin-dependent myosin light chain kinase (MLCK) on the tension-pCa relation of chemically skinned human atrial fibres. MLCK-induced increased myosin P-LC phosphorylation sensitized human atrial skinned fibres for calcium by 0.11 pCa-units in patients with valvular heart disease, and by 0.05 to 0.07 pCa-units in patients with coronary heart disease. The MLCK effect could be antagonized by a light chain phosphatase. The protein phosphatase ocadaic acid (OA) had no influence on the tension-pCa relation of skinned human atrial fibres and had no potentiating effect together with MLCK. The MLCK preparation used in this study was from bovine ventricle and revealed a KM of 1.8 x 10(-5) M and a Vmax of 822 nmol Pi/min/mg using purified bovine ventricular myosin-LCs as substrate.

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Year:  1988        PMID： 3190654     DOI： 10.1007/bf02005820

Source DB:  PubMed          Journal:  Basic Res Cardiol        ISSN： 0300-8428            Impact factor:   17.165


  30 in total

1.  Isolation of cardiac myofibrils and myosin light chains with in vivo levels of light chain phosphorylation.

Authors:  M J Holroyde; D A Small; E Howe; R J Solaro
Journal:  Biochim Biophys Acta       Date:  1979-11-01

2.  Smooth muscle myosin phosphatase inhibition and force enhancement by black sponge toxin.

Authors:  A Takai; C Bialojan; M Troschka; J C Rüegg
Journal:  FEBS Lett       Date:  1987-06-08       Impact factor: 4.124

3.  Frequency-dependent myosin light chain phosphorylation in isolated myocardium.

Authors:  P J Silver; L M Buja; J T Stull
Journal:  J Mol Cell Cardiol       Date:  1986-01       Impact factor: 5.000

4.  The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.

Authors:  R Eisenthal; A Cornish-Bowden
Journal:  Biochem J       Date:  1974-06       Impact factor: 3.857

Review 5.  Modulation of aortic protein phosphatase activity by polylysine.

Authors:  J Di Salvo; D Gifford; A Kokkinakis
Journal:  Proc Soc Exp Biol Med       Date:  1984-10

6.  Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells.

Authors:  A Fabiato; F Fabiato
Journal:  J Physiol (Paris)       Date:  1979

7.  The effect of myosin phosphorylation on the contractile properties of skinned rabbit skeletal muscle fibers.

Authors:  A Persechini; J T Stull; R Cooke
Journal:  J Biol Chem       Date:  1985-07-05       Impact factor: 5.157

8.  Calcium sensitivity of myofilaments in cardiac muscle--effect of myosin phosphorylation.

Authors:  I Morano; J C Rüegg
Journal:  Basic Res Cardiol       Date:  1986       Impact factor: 17.165

9.  Myofibrillar calcium sensitivity modulation: influence of light chain phosphorylation and positive inotropic drugs on skinned frog skeletal muscle.

Authors:  I Morano; G Piazzesi; J C Rüegg
Journal:  Adv Exp Med Biol       Date:  1988       Impact factor: 2.622

10.  Electromechanical effects of okadaic acid isolated from black sponge in guinea-pig ventricular muscles.

Authors:  I Kodama; N Kondo; S Shibata
Journal:  J Physiol       Date:  1986-09       Impact factor: 5.182
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  10 in total

Review 1.  Thick filament proteins and performance in human heart failure.

Authors:  Bradley M Palmer
Journal:  Heart Fail Rev       Date:  2005-09       Impact factor: 4.214

2.  Myosin head orientation: a structural determinant for the Frank-Starling relationship.

Authors:  Gerrie P Farman; David Gore; Edward Allen; Kelly Schoenfelt; Thomas C Irving; Pieter P de Tombe
Journal:  Am J Physiol Heart Circ Physiol       Date:  2011-04-01       Impact factor: 4.733

Review 3.  Signaling to myosin regulatory light chain in sarcomeres.

Authors:  Kristine E Kamm; James T Stull
Journal:  J Biol Chem       Date:  2011-01-21       Impact factor: 5.157

4.  Mechanism of action of endothelin in rat cardiac muscle: cross-bridge kinetics and myosin light chain phosphorylation.

Authors:  G H Rossmanith; J F Hoh; L Turnbull; R I Ludowyke
Journal:  J Physiol       Date:  1997-11-15       Impact factor: 5.182

5.  Regulation of human heart contractility by essential myosin light chain isoforms.

Authors:  M Morano; U Zacharzowski; M Maier; P E Lange; V Alexi-Meskishvili; H Haase; I Morano
Journal:  J Clin Invest       Date:  1996-07-15       Impact factor: 14.808

6.  Role of protein kinase C in the phosphorylation of cardiac myosin light chain 2.

Authors:  R C Venema; R L Raynor; T A Noland; J F Kuo
Journal:  Biochem J       Date:  1993-09-01       Impact factor: 3.857

7.  Ablation of ventricular myosin regulatory light chain phosphorylation in mice causes cardiac dysfunction in situ and affects neighboring myofilament protein phosphorylation.

Authors:  Sarah B Scruggs; Aaron C Hinken; Ariyaporn Thawornkaiwong; Jeffrey Robbins; Lori A Walker; Pieter P de Tombe; David L Geenen; Peter M Buttrick; R John Solaro
Journal:  J Biol Chem       Date:  2008-12-23       Impact factor: 5.157

8.  Myosin P-light chain isoenzymes in the human heart: evidence for diphosphorylation of the atrial P-LC form.

Authors:  I Morano; M Wankerl; M Böhm; E Erdmann; J C Rüegg
Journal:  Basic Res Cardiol       Date:  1989 May-Jun       Impact factor: 17.165

9.  Expression of myosin heavy and light chains and phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the European hamster during hibernation and in summer.

Authors:  I Morano; K Adler; B Agostini; W Hasselbach
Journal:  J Muscle Res Cell Motil       Date:  1992-02       Impact factor: 2.698

10.  Acceleration of stretch activation in murine myocardium due to phosphorylation of myosin regulatory light chain.

Authors:  Julian E Stelzer; Jitandrakumar R Patel; Richard L Moss
Journal:  J Gen Physiol       Date:  2006-08-14       Impact factor: 4.086
  10 in total

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