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Literature DB >> 3189772

Zinc-binding proteins detected by protein blotting.

Abstract

The Western blotting technique was used for the detection of zinc-binding proteins. Proteins were separated electrophoretically on 15% polyacrylamide-sodium dodecyl sulfate minigels, the gels were soaked in a reduction buffer, and the proteins were transferred to nitrocellulose filters. Zinc-binding proteins were probed with radioactive zinc (65Zn) and were detected by autoradiography. This technique allows the detection of as little as 20 to 100 pmol of zinc metalloproteins.

Entities: Chemical

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Year: 1988 PMID： 3189772 DOI： 10.1016/0003-2697(88)90408-3

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

16 in total

1. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.

Authors: Y Cavaloc; C F Bourgeois; L Kister; J Stévenin
Journal: RNA Date: 1999-03 Impact factor: 4.942

2. The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA.

Authors: G Gradwohl; J M Ménissier de Murcia; M Molinete; F Simonin; M Koken; J H Hoeijmakers; G de Murcia
Journal: Proc Natl Acad Sci U S A Date: 1990-04 Impact factor: 11.205

3. The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding.

Authors: S Ahmed; R Kozma; J Lee; C Monfries; N Harden; L Lim
Journal: Biochem J Date: 1991-11-15 Impact factor: 3.857

4. Poly(ADP-ribose)polymerase: a novel finger protein.

Authors: A Mazen; J Menissier-de Murcia; M Molinete; F Simonin; G Gradwohl; G Poirier; G de Murcia
Journal: Nucleic Acids Res Date: 1989-06-26 Impact factor: 16.971

5. Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.

Authors: B Chatton; J L Bocco; M Gaire; C Hauss; B Reimund; J Goetz; C Kedinger
Journal: Mol Cell Biol Date: 1993-01 Impact factor: 4.272

Zinc-binding proteins detected by protein blotting.

1. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.

2. The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA.

3. The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding.

4. Poly(ADP-ribose)polymerase: a novel finger protein.

5. Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.

6. Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II.

7. Myelin basic protein is a zinc-binding protein in brain: possible role in myelin compaction.

Review 8. Structure and function of poly(ADP-ribose) polymerase.

9. Cadmium speciation studies in the intestine of Lumbricus terrestris by electrophoresis of metal proteins complexes.

10. Kin17, a mouse nuclear zinc finger protein that binds preferentially to curved DNA.