Literature DB >> 318934

Automated computer analysis for enzyme-multiplied immunological techniques.

D Rodbard, S W McClean.   

Abstract

The dose/response curve in enzyme-multiplied immunoassays (EMIT) and related techniques may be described by the same "four-parameter logistic function" as has proven useful in radioimmunoassays, immunoradiometric assays (IRMA), and two-site immunoradiometric ("sandwhich") assays. This function provides an empirical description of the dose/response curve by use of an objective least-squares regression analysis, with the advantages of computerization and automation. This facilitates further statistical analyses, quality control, estimation of precision for an unknown, and improved, objective estimation of the sensitivity or minimal detectable dose.

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Year:  1977        PMID: 318934

Source DB:  PubMed          Journal:  Clin Chem        ISSN: 0009-9147            Impact factor:   8.327


  10 in total

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2.  Antibody response in group B meningococcal disease determined by enzyme-linked immunosorbent assay with serotype 15 outer membrane antigen.

Authors:  S Harthug; E Rosenqvist; E A Høiby; T W Gedde-Dahl; L O Frøholm
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

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Authors:  M Van der Giessen; A P van den Berg; W van der Bij; S Postma; W J van Son; T H The
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Review 4.  Progress in enzyme immunoassays: production of reagents, experimental design, and interpretation.

Authors:  E Kurstak
Journal:  Bull World Health Organ       Date:  1985       Impact factor: 9.408

5.  Potential liabilities of gentamicin homogeneous enzyme immunoassay.

Authors:  L G Carlson; C J Delaney; J J Plorde
Journal:  Antimicrob Agents Chemother       Date:  1982-01       Impact factor: 5.191

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7.  Metabolism of retinoic acid and retinol during differentiation of F9 embryonal carcinoma cells.

Authors:  J B Williams; J L Napoli
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Journal:  Epidemiol Infect       Date:  1992-10       Impact factor: 2.451

9.  In vivo clearance studies of the terminal fluid-phase complement complex in rabbits.

Authors:  F Hugo; C Berstecher; S Krämer; W Fassbender; S Bhakdi
Journal:  Clin Exp Immunol       Date:  1989-07       Impact factor: 4.330

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Authors:  Kala Natarajan; James W Overstreet; Jane M Rogers; Michael S Denison; Jiangang Chen; Peter N Lohstroh; Daniel S McConnell; Bill L Lasley
Journal:  Environ Health Perspect       Date:  2002-08       Impact factor: 9.031

  10 in total

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