Literature DB >> 31891428

Hexameric structure of the ATPase motor subunit of magnesium chelatase in chlorophyll biosynthesis.

Yong-Shan Gao1, Yan-Li Wang1, Xiao Wang1, Lin Liu1.   

Abstract

Magnesium chelatase (MgCh) is a heterotrimeric enzyme complex, composed of two AAA+ family subunits that can assembly into a double ring structure and a large catalytic subunit. The small AAA+ subunit has ATPase activity and can self-oligomerize into a ring structure, while the other AAA+ subunit lacks independent ATPase activity. Previous structural studies of the ATPase motor subunit of MgCh from a bacteriochlorophyll-synthesizing bacterium have identified a unique ATPase clade, but the model of oligomeric assembly is unclear. Here we present the hexameric structure of the MgCh ATPase motor subunit from the chlorophyll-synthesizing cyanobacterium Synechocystis sp. PCC 6803. This structure reveals details of how the hexameric ring is assembled, and thus provides a basis for further studying the heterotrimeric complex.
© 2019 The Protein Society.

Entities:  

Keywords:  zzm321990Synechocystis; AAA+ ring hexamer; ATPase; chlorophyll biosynthesis; magnesium chelatase; motor

Mesh：

Substances：

Year:  2020        PMID： 31891428      PMCID： PMC7096714          DOI： 10.1002/pro.3816

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  40 in total

1.  ATPase activity associated with the magnesium-protoporphyrin IX chelatase enzyme of Synechocystis PCC6803: evidence for ATP hydrolysis during Mg2+ insertion, and the MgATP-dependent interaction of the ChlI and ChlD subunits.

Authors:  P E Jensen; L C Gibson; C N Hunter
Journal:  Biochem J       Date:  1999-04-01       Impact factor: 3.857

2.  Three semidominant barley mutants with single amino acid substitutions in the smallest magnesium chelatase subunit form defective AAA+ hexamers.

Authors:  A Hansson; R D Willows; T H Roberts; M Hansson
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-30       Impact factor: 11.205

3.  Chelatases: distort to select?

Authors:  Salam Al-Karadaghi; Ricardo Franco; Mats Hansson; John A Shelnutt; Grazia Isaya; Gloria C Ferreira
Journal:  Trends Biochem Sci       Date:  2006-02-15       Impact factor: 13.807

4.  Kinetic analyses of the magnesium chelatase provide insights into the mechanism, structure, and formation of the complex.

Authors:  Artur Sawicki; Robert D Willows
Journal:  J Biol Chem       Date:  2008-09-12       Impact factor: 5.157

5.  ATP-induced conformational dynamics in the AAA+ motor unit of magnesium chelatase.

Authors:  Joakim Lundqvist; Hans Elmlund; Ragna Peterson Wulff; Lisa Berglund; Dominika Elmlund; Cecilia Emanuelsson; Hans Hebert; Robert D Willows; Mats Hansson; Martin Lindahl; Salam Al-Karadaghi
Journal:  Structure       Date:  2010-03-10       Impact factor: 5.006

6.  Determinants of catalytic activity with the use of purified I, D and H subunits of the magnesium protoporphyrin IX chelatase from Synechocystis PCC6803.

Authors:  P E Jensen; L C Gibson; C N Hunter
Journal:  Biochem J       Date:  1998-09-01       Impact factor: 3.857

7.  EM single particle analysis of the ATP-dependent BchI complex of magnesium chelatase: an AAA+ hexamer.

Authors:  R D Willows; A Hansson; D Birch; S Al-Karadaghi; M Hansson
Journal:  J Struct Biol       Date:  2004 Apr-May       Impact factor: 2.867

8.  Overview of the CCP4 suite and current developments.

Authors:  Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18

9.  MolProbity: all-atom structure validation for macromolecular crystallography.

Authors:  Vincent B Chen; W Bryan Arendall; Jeffrey J Headd; Daniel A Keedy; Robert M Immormino; Gary J Kapral; Laura W Murray; Jane S Richardson; David C Richardson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-12-21

10.  Nanomechanical and Thermophoretic Analyses of the Nucleotide-Dependent Interactions between the AAA(+) Subunits of Magnesium Chelatase.

Authors:  Nathan B P Adams; Cvetelin Vasilev; Amanda A Brindley; C Neil Hunter
Journal:  J Am Chem Soc       Date:  2016-05-12       Impact factor: 15.419
View more
  7 in total

1.  Hexameric structure of the ATPase motor subunit of magnesium chelatase in chlorophyll biosynthesis.

Authors:  Yong-Shan Gao; Yan-Li Wang; Xiao Wang; Lin Liu
Journal:  Protein Sci       Date:  2020-01-07       Impact factor: 6.725

2.  Bilin-dependent regulation of chlorophyll biosynthesis by GUN4.

Authors:  Weiqing Zhang; Robert D Willows; Rui Deng; Zheng Li; Mengqi Li; Yan Wang; Yunling Guo; Weida Shi; Qiuling Fan; Shelley S Martin; Nathan C Rockwell; J Clark Lagarias; Deqiang Duanmu
Journal:  Proc Natl Acad Sci U S A       Date:  2021-05-18       Impact factor: 11.205

3.  Genomic regions associated with physiological, biochemical and yield-related responses under water deficit in diploid potato at the tuber initiation stage revealed by GWAS.

Authors:  Paula Díaz; Felipe Sarmiento; Boby Mathew; Agim Ballvora; Teresa Mosquera Vásquez
Journal:  PLoS One       Date:  2021-11-08       Impact factor: 3.240

4.  Impact of Porphyrin Binding to GENOMES UNCOUPLED 4 on Tetrapyrrole Biosynthesis in planta.

Authors:  Vincent Fölsche; Christopher Großmann; Andreas S Richter
Journal:  Front Plant Sci       Date:  2022-03-15       Impact factor: 5.753

5.  Chlorophyll decomposition is accelerated in banana leaves after the long-term magnesium deficiency according to transcriptome analysis.

Authors:  Baolin Kan; Yong Yang; Pengmeng Du; Xinping Li; Wenjie Lai; Haiyan Hu
Journal:  PLoS One       Date:  2022-06-24       Impact factor: 3.752

Review 6.  The requirement for cobalt in vitamin B12: A paradigm for protein metalation.

Authors:  Deenah Osman; Anastasia Cooke; Tessa R Young; Evelyne Deery; Nigel J Robinson; Martin J Warren
Journal:  Biochim Biophys Acta Mol Cell Res       Date:  2020-10-21       Impact factor: 4.739

7.  Heterologous Expression of the Barley (Hordeum vulgare L.) Xantha-f, -g and -h Genes that Encode Magnesium Chelatase Subunits.

Authors:  Rabab Mahdi; David Stuart; Mats Hansson; Helmy M Youssef
Journal:  Protein J       Date:  2020-10       Impact factor: 2.371
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.