Frank Mayta-Tovalino1, Gabriela Sedano-Balbin2, Percy Romero-Tapia2, Daniel Alvítez-Temoche3, María Álvarez-Paucar4, Luis Gálvez-Calla5, Sonia Sacsaquispe-Contreras5. 1. Postgraduate Department, School of Stomatology, Universidad Científica del Sur, Peru, Phone: +51 1 214-2500, e-mail: fmaytat@ucientifica.edu.pe. 2. Academic Department of Stomatology Medical Surgical, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru. 3. Academic Department of Stomatology, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima, Peru. 4. Department of Basic Sciences, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru. 5. Academic Department of Medicine and Buccomaxillofacial Surgery, Faculty of Stomatology, Universidad Peruana Cayetano Heredia, Lima, Peru.
Abstract
AIM: "Tocosh" is a potato that has undergone a process of hydraulic oxidation that enhances its antimicrobial properties so that this natural resource can be used in medical sciences. The aim of this study was to develop and evaluate the antibacterial and cytotoxic activity of a new experimental tooth based on Solanum tuberosum "Tocosh" on the cell lines 3T3 and DU145. MATERIALS AND METHODS: To evaluate the cytotoxicity, cell cultures 3T3 and DU145 were used. Cell viability was determined by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in a medium containing 10% calf serum where the cells were preincubated at a concentration of 1 × 106 cells/mL in culture medium for 3 hours at 37°C and 6.5% CO2. Then, the absorbance was measured using a microplate reader where the formazan crystals were diluted with acidic and cold isopropanol, and quantified in an ELISA reader. To evaluate the antibacterial effect, the Kirby Bauer inhibition halos method was used on strains of S. aureus (ATCC 25923), S. mutans (ATCC 25175), and S. mitis (ATCC 49456). RESULTS: Solanum tuberosum (tocosh) was not cytotoxic because it only had one CC50 at the concentration of 0.26927 mg/mL and 0.26845 mg/mL for the cell lines 3T3 and DU145, respectively. Tocosh toothpaste (TD) has an antibacterial effect against S. aureus and S. mutans. CONCLUSION: The new ecological dentifrice was not cytotoxic since it did not alter cell viability because its CC50 was only 0.268 and 0.269 μg/mL for the 3T3 and DU145 cell lines, respectively; however, it presented an optimal antimicrobial activity against the oral strains evaluated. CLINICAL SIGNIFICANCE: This research has great potential for clinical use because this new dentifrice has antimicrobial activity against different oral germs. How to cite this article: Mayta-Tovalino F, Sedano-Balbin G, Romero-Tapia P, et al. Development of New Experimental Dentifrice of Peruvian Solanum tuberosum (Tocosh) Fermented by Water Stress: Antibacterial and Cytotoxic Activity. J Contemp Dent Pract 2019;20(10):1206-1211.
AIM: "Tocosh" is a potato that has undergone a process of hydraulic oxidation that enhances its antimicrobial properties so that this natural resource can be used in medical sciences. The aim of this study was to develop and evaluate the antibacterial and cytotoxic activity of a new experimental tooth based on Solanum tuberosum "Tocosh" on the cell lines 3T3 and DU145. MATERIALS AND METHODS: To evaluate the cytotoxicity, cell cultures 3T3 and DU145 were used. Cell viability was determined by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in a medium containing 10% calf serum where the cells were preincubated at a concentration of 1 × 106 cells/mL in culture medium for 3 hours at 37°C and 6.5% CO2. Then, the absorbance was measured using a microplate reader where the formazan crystals were diluted with acidic and cold isopropanol, and quantified in an ELISA reader. To evaluate the antibacterial effect, the Kirby Bauer inhibition halos method was used on strains of S. aureus (ATCC 25923), S. mutans (ATCC 25175), and S. mitis (ATCC 49456). RESULTS:Solanum tuberosum (tocosh) was not cytotoxic because it only had one CC50 at the concentration of 0.26927 mg/mL and 0.26845 mg/mL for the cell lines 3T3 and DU145, respectively. Tocosh toothpaste (TD) has an antibacterial effect against S. aureus and S. mutans. CONCLUSION: The new ecological dentifrice was not cytotoxic since it did not alter cell viability because its CC50 was only 0.268 and 0.269 μg/mL for the 3T3 and DU145 cell lines, respectively; however, it presented an optimal antimicrobial activity against the oral strains evaluated. CLINICAL SIGNIFICANCE: This research has great potential for clinical use because this new dentifrice has antimicrobial activity against different oral germs. How to cite this article: Mayta-Tovalino F, Sedano-Balbin G, Romero-Tapia P, et al. Development of New Experimental Dentifrice of Peruvian Solanum tuberosum (Tocosh) Fermented by Water Stress: Antibacterial and Cytotoxic Activity. J Contemp Dent Pract 2019;20(10):1206-1211.
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