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Literature DB >> 3186439

A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.

Y N Xia¹, R Morgan, I Schildkraut, J L Van Etten.

Abstract

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

Entities: Disease Gene

Mesh：

Substances：

Year: 1988 PMID： 3186439 PMCID： PMC338757 DOI： 10.1093/nar/16.20.9477

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. The cleavage site of the RsaI isoschizomer, CviII, is G decreases TAC.

Authors: Y N Xia; K E Narva; J L Van Etten
Journal: Nucleic Acids Res Date: 1987-12-10 Impact factor: 16.971

2. Lytic viruses infecting a Chlorella-like alga.

Authors: J L Van Etten; D E Burbank; A M Schuster; R H Meints
Journal: Virology Date: 1985-01-15 Impact factor: 3.616

3. Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA.

Authors: S G Rogers; M Rhoades
Journal: Proc Natl Acad Sci U S A Date: 1976-05 Impact factor: 11.205

4. A site-specific single-strand endonuclease from the eukaryote Chlamydomonas.

Authors: W G Burton; R J Roberts; P A Myers; R Sager
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

5. Insertion mutations in the dam gene of Escherichia coli K-12.

Authors: M G Marinus; M Carraway; A Z Frey; L Brown; J A Arraj
Journal: Mol Gen Genet Date: 1983

6. Assaying of organisms for the presence of restriction endonucleases.

Authors: R Schleif
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. Separation and isolation of DNA fragments using linear polyacrylamide gradient gel electrophoresis.

Authors: P G Jeppesen
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

8. Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius.

Authors: R W Blakesley; J B Dodgson; I F Nes; R D Wells
Journal: J Biol Chem Date: 1977-10-25 Impact factor: 5.157

9. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1967-06 Impact factor: 3.162

10. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

18 in total

1. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M McClelland; M Nelson
Journal: Nucleic Acids Res Date: 1992-05-11 Impact factor: 16.971

2. Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.

Authors: C Stefan; Y N Xia; J L Van Etten
Journal: Nucleic Acids Res Date: 1991-01-25 Impact factor: 16.971

3. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

Review 4. Viruses and viruslike particles of eukaryotic algae.

Authors: J L Van Etten; L C Lane; R H Meints
Journal: Microbiol Rev Date: 1991-12

5. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1989 Impact factor: 16.971

6. Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A.

Authors: Lisa A Fitzgerald; Michael V Graves; Xiao Li; Tamara Feldblyum; William C Nierman; James L Van Etten
Journal: Virology Date: 2006-10-04 Impact factor: 3.616

7. Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification.

Authors: Siu-hong Chan; Zhenyu Zhu; James L Van Etten; Shuang-yong Xu
Journal: Nucleic Acids Res Date: 2004-11-29 Impact factor: 16.971