Tatjana Varljen1, Gordana Sekulovic2, Olgica Rakic2, Nela Maksimovic3, Biljana Jekic3, Ivana Novakovic3, Tatjana Damnjanovic4. 1. Institute of Legal Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia. 2. Institute of Neonatology, Belgrade, Serbia. 3. Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia. 4. Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia. tatjanadamnjanovic@yahoo.com.
Abstract
OBJECTIVE: Interleukin-1-B (IL1B) is a proinflammatory cytokine that plays an important role in sepsis. The aim of this study was to evaluate the relationships between IL1B-511G/A polymorphism and susceptibility and outcome of early-onset sepsis (EOS) in preterm infants. METHODS: DNA was extracted from the buccal swabs of 471 (285 with EOS and 186 control) preterm infants. Genotypes of rs16944 polymorphism were determined with real-time PCR method. RESULTS: We found statistically significant higher frequency of IL1B-511AA genotype in EOS group than in control group (p = 0.012). Also, IL1B-511AA genotype is statistically significantly more frequent in patients with lethal EOS outcome (p = 0.011). CONCLUSION: Genotype IL1B-511AA was associated with susceptibility to EOS and it is a significant predictor of lethal outcome in preterm infants with EOS.
OBJECTIVE: Interleukin-1-B (IL1B) is a proinflammatory cytokine that plays an important role in sepsis. The aim of this study was to evaluate the relationships between IL1B-511G/A polymorphism and susceptibility and outcome of early-onset sepsis (EOS) in preterm infants. METHODS: DNA was extracted from the buccal swabs of 471 (285 with EOS and 186 control) preterm infants. Genotypes of rs16944 polymorphism were determined with real-time PCR method. RESULTS: We found statistically significant higher frequency of IL1B-511AA genotype in EOS group than in control group (p = 0.012). Also, IL1B-511AA genotype is statistically significantly more frequent in patients with lethal EOS outcome (p = 0.011). CONCLUSION: Genotype IL1B-511AA was associated with susceptibility to EOS and it is a significant predictor of lethal outcome in preterm infants with EOS.