Munsoor A Hanifa1,2,3, Raluca G Maltesen2, Bodil S Rasmussen2,3, Katrine B Buggeskov4, Hanne B Ravn4, Martin Skott5, Søren Nielsen6, Jørgen Frøkiær7, Troels Ring8,9, Reinhard Wimmer10. 1. Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark. 2. Department of Anesthesia and Intensive Care Medicine, Aalborg University Hospital, 9000, Aalborg, Denmark. 3. Department of Clinical Medicine, Aalborg University, 9000, Aalborg, Denmark. 4. Department of Cardiothoracic Anesthesiology, Rigshospitalet, Copenhagen University Hospital, 2100, Copenhagen, Denmark. 5. Department of Urology, Aarhus University Hospital, 8250, Aarhus N, Denmark. 6. Meta-IQ, Fredensgade 9, 8000, Aarhus C, Denmark. 7. Department of Clinical Medicine, Aarhus University, 8200, Aarhus N, Denmark. 8. Department of Biomedicine, Aarhus University, 8000, Aarhus C, Denmark. 9. The Center for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh, Pittsburg, PA, 15261, USA. 10. Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark. rw@bio.aau.dk.
Abstract
BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.
BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.
Authors: Joana Pinto; M Rosário M Domingues; Eulália Galhano; Cristina Pita; Maria do Céu Almeida; Isabel M Carreira; Ana M Gil Journal: Analyst Date: 2014-01-20 Impact factor: 4.616
Authors: Olaf Beckonert; Hector C Keun; Timothy M D Ebbels; Jacob Bundy; Elaine Holmes; John C Lindon; Jeremy K Nicholson Journal: Nat Protoc Date: 2007 Impact factor: 13.491
Authors: Anthony C Dona; Beatriz Jiménez; Hartmut Schäfer; Eberhard Humpfer; Manfred Spraul; Matthew R Lewis; Jake T M Pearce; Elaine Holmes; John C Lindon; Jeremy K Nicholson Journal: Anal Chem Date: 2014-09-16 Impact factor: 6.986
Authors: Katrine B Buggeskov; Raluca G Maltesen; Bodil S Rasmussen; Munsoor A Hanifa; Morten A V Lund; Reinhard Wimmer; Hanne B Ravn Journal: J Clin Med Date: 2018-11-21 Impact factor: 4.241