Mandana Beigi Boroujeni1,2, Fatemeh Peidayesh3, Afshin Pirnia1, Nasim Beigi Boroujeni1, Seyyed Amir Yasin Ahmadi4, Mohammadreza Gholami5. 1. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. 2. Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran. 3. Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran. 4. Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran. 5. Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract
BACKGROUND: During treatment of childhood cancers, fertility of boys may be affected. Therefore, freezing spermatogonial stem cell (SSC) is recommended. However, freezing-thawing process may cause damage to SSCs. This study was conducted to evaluate protective effects of selenium on freezing-thawing damage of mice SSCs using investigation of cell viability and investigation of apoptosis related genes expression including Fas, Caspase3, Bcl2, Bax and P53. METHODS: SSCs were extracted from 80 6-day-old mice. The SSCs were divided into four groups: cryopreservation along with selenium (low and high dose), vitrification along with selenium (low and high dose), cryopreservation control, and vitrification control. Trypan blue staining and real-time polymerase chain reaction (real-time PCR) were used to investigate cell viability and gene expression, respectively. RESULT: Comparison of cell viability in the experimental groups did not show a significant association. Expression of Fas and Caspase3 was significantly lower in cryopreservation group with low-dose selenium. Expression of Bcl2 was significantly lower in cryopreservation group with high-dose selenium. Expression of Bax and Caspase3 was significantly lower in vitrification group with low-dose selenium, and expression of P53 was significantly upper. Expression of Bax and Fas was significantly lower in vitrification group with high-dose selenium, and expression of P53 was significantly upper (P<0.001). CONCLUSIONS: Selenium had dose dependent effect on apoptosis related genes profile. The only evident effect was the effect of low-dose selenium in cryopreservation on inhibition of apoptosis via extrinsic pathway. 2019 Stem Cell Investigation. All rights reserved.
BACKGROUND: During treatment of childhood cancers, fertility of boys may be affected. Therefore, freezing spermatogonial stem cell (SSC) is recommended. However, freezing-thawing process may cause damage to SSCs. This study was conducted to evaluate protective effects of selenium on freezing-thawing damage of mice SSCs using investigation of cell viability and investigation of apoptosis related genes expression including Fas, Caspase3, Bcl2, Bax and P53. METHODS: SSCs were extracted from 80 6-day-old mice. The SSCs were divided into four groups: cryopreservation along with selenium (low and high dose), vitrification along with selenium (low and high dose), cryopreservation control, and vitrification control. Trypan blue staining and real-time polymerase chain reaction (real-time PCR) were used to investigate cell viability and gene expression, respectively. RESULT: Comparison of cell viability in the experimental groups did not show a significant association. Expression of Fas and Caspase3 was significantly lower in cryopreservation group with low-dose selenium. Expression of Bcl2 was significantly lower in cryopreservation group with high-dose selenium. Expression of Bax and Caspase3 was significantly lower in vitrification group with low-dose selenium, and expression of P53 was significantly upper. Expression of Bax and Fas was significantly lower in vitrification group with high-dose selenium, and expression of P53 was significantly upper (P<0.001). CONCLUSIONS: Selenium had dose dependent effect on apoptosis related genes profile. The only evident effect was the effect of low-dose selenium in cryopreservation on inhibition of apoptosis via extrinsic pathway. 2019 Stem Cell Investigation. All rights reserved.
Authors: Hanna Valli; Meena Sukhwani; Serena L Dovey; Karen A Peters; Julia Donohue; Carlos A Castro; Tianjiao Chu; Gary R Marshall; Kyle E Orwig Journal: Fertil Steril Date: 2014-06-02 Impact factor: 7.329
Authors: Marco Ghezzi; Massimiliano Berretta; Alberto Bottacin; Pierfrancesco Palego; Barbara Sartini; Ilaria Cosci; Livio Finos; Riccardo Selice; Carlo Foresta; Andrea Garolla Journal: Front Pharmacol Date: 2016-05-13 Impact factor: 5.810