Ítallo Emídio Lira Viana1, Raquel Marianna Lopes2, Flávia Rodrigues Oliveira Silva3, Nelson Batista Lima4, Ana Cecília Corrêa Aranha5, Sabrina Feitosa6, Taís Scaramucci7. 1. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, São Paulo, SP, 05508-000, Brazil. Electronic address: italloviana@usp.br. 2. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, São Paulo, SP, 05508-000, Brazil. Electronic address: quelmlopes@gmail.com. 3. Nuclear and Energy Research Institute (IPEN-CNEN), Material Science and Technology Center, Av. Prof. Lineu Prestes 2242, São Paulo, SP, 05508-000, Brazil. Electronic address: frsilva@ipen.br. 4. Nuclear and Energy Research Institute (IPEN-CNEN), Material Science and Technology Center, Av. Prof. Lineu Prestes 2242, São Paulo, SP, 05508-000, Brazil. Electronic address: nblima22@gmail.com. 5. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, São Paulo, SP, 05508-000, Brazil. Electronic address: acca@usp.br. 6. Clinical Assistant Professor, Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, 1121 Michigan Street, Indianapolis, IN, 46202-5186, United States. Electronic address: sfeitosa@iu.edu. 7. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, São Paulo, SP, 05508-000, Brazil. Electronic address: tais.sca@usp.br.
Abstract
OBJECTIVE: To evaluate the anti-erosive effect of solutions containing β-tricalcium phosphate (β-TCP) nanoparticles functionalized with fluoride or with fluoride plus stannous on enamel and dentin. METHODS: β-TCP nanoparticles were synthesized and characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Sixty enamel and dentin specimens were randomly allocated into the groups (n = 10): Control (water); F (NaF, 225 ppm F-); F + Sn (NaF + SnCl2, 800 ppm Sn2+); F+β-TCP (F+40 ppm β-TCP); F + Sn+β-TCP (F + Sn+40 ppm β-TCP); F + Sn+100β-TCP (F + Sn+100 ppm β-TCP). Specimens underwent erosion-remineralization cycling (5 min immersion into 1% citric acid solution and 60 min exposure to artificial saliva, 4×/day, 5 days). Immersion in the test solutions was performed for 2 min, 2×/day. Surface loss (SL, in µm) was determined by optical profilometry at the end of cycling. Data were analyzed using one way-ANOVA and Tukey's tests (α = 0.05). RESULTS: XRD confirmed the β-TCP phase. TEM micrographs showed differences between the bare nanoparticle and the β-TCP functionalized with F and Sn. All enamel groups presented lower SL than the control, with F + Sn, F + Sn+β-TCP, and F + Sn+100β-TCP showing the lowest values. For dentin, all the groups had lower SL than the control. F+β-TCP presented the lowest SL, significantly differing from all the other groups. CONCLUSION: β-TCP nanoparticles functionalized with fluoride showed improved anti-erosive effect compared to the fluoride solution on dentin. There was no significant effect of the β-TCP nanoparticles functionalized with fluoride plus stannous in both substrates. CLINICAL RELEVANCE: β-TCP nanoparticles are a promising agent to be added to oral health products to improve the protective effect of fluoride against dentin erosion.
OBJECTIVE: To evaluate the anti-erosive effect of solutions containing β-tricalcium phosphate (β-TCP) nanoparticles functionalized with fluoride or with fluoride plus stannous on enamel and dentin. METHODS: β-TCP nanoparticles were synthesized and characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Sixty enamel and dentin specimens were randomly allocated into the groups (n = 10): Control (water); F (NaF, 225 ppm F-); F + Sn (NaF + SnCl2, 800 ppm Sn2+); F+β-TCP (F+40 ppm β-TCP); F + Sn+β-TCP (F + Sn+40 ppm β-TCP); F + Sn+100β-TCP (F + Sn+100 ppm β-TCP). Specimens underwent erosion-remineralization cycling (5 min immersion into 1% citric acid solution and 60 min exposure to artificial saliva, 4×/day, 5 days). Immersion in the test solutions was performed for 2 min, 2×/day. Surface loss (SL, in µm) was determined by optical profilometry at the end of cycling. Data were analyzed using one way-ANOVA and Tukey's tests (α = 0.05). RESULTS: XRD confirmed the β-TCP phase. TEM micrographs showed differences between the bare nanoparticle and the β-TCP functionalized with F and Sn. All enamel groups presented lower SL than the control, with F + Sn, F + Sn+β-TCP, and F + Sn+100β-TCP showing the lowest values. For dentin, all the groups had lower SL than the control. F+β-TCP presented the lowest SL, significantly differing from all the other groups. CONCLUSION: β-TCP nanoparticles functionalized with fluoride showed improved anti-erosive effect compared to the fluoride solution on dentin. There was no significant effect of the β-TCP nanoparticles functionalized with fluoride plus stannous in both substrates. CLINICAL RELEVANCE: β-TCP nanoparticles are a promising agent to be added to oral health products to improve the protective effect of fluoride against dentin erosion.
Authors: Inna V Fadeeva; Margarita A Goldberg; Ilya I Preobrazhensky; Georgy V Mamin; Galina A Davidova; Nadezhda V Agafonova; Marco Fosca; Fabrizio Russo; Sergey M Barinov; Simona Cavalu; Julietta V Rau Journal: J Mater Sci Mater Med Date: 2021-08-18 Impact factor: 3.896
Authors: Andrea Butera; Simone Gallo; Maurizio Pascadopoli; Giuseppe Alessandro Scardina; Sofia Pezzullo; Andrea Scribante Journal: J Clin Med Date: 2022-08-20 Impact factor: 4.964