A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression.

Here, we report a novel dual-functional microRNA (miRNA) probe, PA-miRNA, for miRNA target identification and light control of miRNA target expression. PA-miRNA is a miRNA mimic with a 3'-biotin tag linked via a photo-cleavable linker. Using PA-miR-34a, intracellular targets of miR-34a in HeLa cells were isolated and confirmed. Moreover, PA-miR-34a upon transfection into HeLa cells was inactive until light irradiation to break the photo-cleavable linker to release functional miR-34a. We demonstrated that miR-34a target expression as well as miR-34a-promoted cell apoptosis were regulated by PA-miR-34a in a photo-controllable manner.