Camilla Vieira Esteves1,2, Wladimir Gushiken de Campos3, Juliana Amorim Dos Santos4, Solange Kobayashi Velasco3, Eliete Neves Silva Guerra4, Walter L Siqueira5, Celso Augusto Lemos3. 1. Department of Oral Medicine, Faculdade de Odontologia, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Butantã, São Paulo, SP, 05508-000, Brazil. camilla.santos@usp.br. 2. College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada. camilla.santos@usp.br. 3. Department of Oral Medicine, Faculdade de Odontologia, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Butantã, São Paulo, SP, 05508-000, Brazil. 4. Faculty of Health Sciences, Laboratory of Oral Histopathology , Universidade de Brasília UNB, Brasília, DF, Brazil. 5. College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada.
Abstract
OBJECTIVE: To present a systematic review that provides updated information about proteins found in salivary fluid extracted strictly from ducts. METHODS: The systematic review probing strategy was based on electronic databases word search (PubMed, EMBASE, LILACS, Web of Science, and Scopus). Risk of bias was assessed based on Joanna Briggs Institute Critical Appraisal Checklist for Studies Reporting Prevalence Data. RESULTS: After 2 rounds of scrutiny, 12 articles were included, totaling 231 individuals (125 were healthy, 41 were elder individuals with radicular caries, 56 had primary Sjögren's syndrome, and 9 were patients who had received radiotherapy for head and neck cancer). The selected studies had no similarities among proteins found, demonstrating the need of standard reference in experimental methodology to obtain a thorough coverage of proteins. CONCLUSION: Further studies are required to better determine the relative amount of proteins described in this study. It is essential to increase the number of samples, to perform similar collection techniques, to include other analyses methods such as mass spectrometry, and to perform the validation of some proteins using immunoassay techniques such as Elisa and Western blot. CLINICAL RELEVANCE: Proteomic profile of saliva collected from ducts is essential to better understand the disease process, enabling the identification of biomarkers for specific clinical situations.
OBJECTIVE: To present a systematic review that provides updated information about proteins found in salivary fluid extracted strictly from ducts. METHODS: The systematic review probing strategy was based on electronic databases word search (PubMed, EMBASE, LILACS, Web of Science, and Scopus). Risk of bias was assessed based on Joanna Briggs Institute Critical Appraisal Checklist for Studies Reporting Prevalence Data. RESULTS: After 2 rounds of scrutiny, 12 articles were included, totaling 231 individuals (125 were healthy, 41 were elder individuals with radicular caries, 56 had primary Sjögren's syndrome, and 9 were patients who had received radiotherapy for head and neck cancer). The selected studies had no similarities among proteins found, demonstrating the need of standard reference in experimental methodology to obtain a thorough coverage of proteins. CONCLUSION: Further studies are required to better determine the relative amount of proteins described in this study. It is essential to increase the number of samples, to perform similar collection techniques, to include other analyses methods such as mass spectrometry, and to perform the validation of some proteins using immunoassay techniques such as Elisa and Western blot. CLINICAL RELEVANCE: Proteomic profile of saliva collected from ducts is essential to better understand the disease process, enabling the identification of biomarkers for specific clinical situations.
Entities:
Keywords:
Duct saliva; Mass Spectrometry; Proteomics
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