Sara Bahonar1,2, Maryam Ghazvinian1, Mohamad Reza Haghshenas1, Hamid Reza Goli1, Bahman Mirzaei1,2. 1. Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. 2. Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran.
Abstract
BACKGROUND: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals. METHODS: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host. RESULTS: LPurification of the macromolecules was confirmed by FTIR and Western blotting. CONCLUSION: The S. epidermidis SesC protein and PIA were uccessfully purified. Both are considered as vaccine candidates.
BACKGROUND: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals. METHODS: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host. RESULTS: LPurification of the macromolecules was confirmed by FTIR and Western blotting. CONCLUSION: The S. epidermidis SesC protein and PIA were uccessfully purified. Both are considered as vaccine candidates.
Authors: Mohammad Shahrooei; Vishal Hira; Laleh Khodaparast; Ladan Khodaparast; Benoit Stijlemans; Soňa Kucharíková; Peter Burghout; Peter W M Hermans; Johan Van Eldere Journal: Infect Immun Date: 2012-07-16 Impact factor: 3.441
Authors: Taisir Saber; Mohamed Samir; Rasha M El-Mekkawy; Eman Ariny; Sara Ramadan El-Sayed; Gamal Enan; Sawasn H Abdelatif; Ahmed Askora; Abdallah M A Merwad; Yasmine H Tartor Journal: Front Microbiol Date: 2022-02-08 Impact factor: 5.640