Haruka Abe1, Yuri Ushijima1, Marguerite M Loembe2, Rodrigue Bikangui3, Georgelin Nguema-Ondo4, Patrick I Mpingabo1, Vahid R Zadeh1, Christelle M Pemba1, Yohei Kurosaki1, Yui Igasaki1, Sophia G de Vries5, Martin P Grobusch6, Selidji T Agnandji3, Bertrand Lell7, Jiro Yasuda8. 1. Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan. 2. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon; Université des Sciences de la Santé de Libreville, Libreville, Gabon. 3. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon; Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany. 4. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon. 5. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon; Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands. 6. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon; Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany; Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands. 7. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon; Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany; Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Vienna, Austria. 8. Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; National Research Center for the Control and Prevention of Infectious Diseases (CCPID), Nagasaki University, Nagasaki, Japan; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. Electronic address: j-yasuda@nagasaki-u.ac.jp.
Abstract
OBJECTIVES: Dengue outbreaks, mainly caused by dengue virus serotype 2 (DENV-2), occurred in 2007 and in 2010 in Gabon, Central Africa. However, information on DENV infections has been insufficient since 2010. The aim of this study was to investigate the current DENV infection scenario and the risk of repeated infections in Gabon. METHODS: During 2015-2017, serum samples were collected from enrolled febrile participants and were tested for DENV infection using RT-qPCR. DENV-positive samples were analyzed for a history of previous DENV infection(s) using ELISA. The complete DENV genome was sequenced to analyze the phylogeny of Gabonese DENV strains. RESULTS: DENV-3 was exclusively detected, with a high rate of anti-DENV IgG seropositivity among DENV-3-positive participants. DENV-3 showed higher infection rates in adults and the infection was seasonal with peaks in the rainy seasons. Phylogenetic analysis revealed that Gabonese DENV-3 originated from West African strains and has been circulating continuously in Gabon since at least 2010, when the first DENV-3 case was reported. CONCLUSIONS: These findings indicate stable DENV-3 circulation and the risk of repeated DENV infections in Gabon, highlighting the need for continuous monitoring to control DENV infections.
OBJECTIVES: Dengue outbreaks, mainly caused by dengue virus serotype 2 (DENV-2), occurred in 2007 and in 2010 in Gabon, Central Africa. However, information on DENV infections has been insufficient since 2010. The aim of this study was to investigate the current DENV infection scenario and the risk of repeated infections in Gabon. METHODS: During 2015-2017, serum samples were collected from enrolled febrile participants and were tested for DENV infection using RT-qPCR. DENV-positive samples were analyzed for a history of previous DENV infection(s) using ELISA. The complete DENV genome was sequenced to analyze the phylogeny of Gabonese DENV strains. RESULTS: DENV-3 was exclusively detected, with a high rate of anti-DENV IgG seropositivity among DENV-3-positive participants. DENV-3 showed higher infection rates in adults and the infection was seasonal with peaks in the rainy seasons. Phylogenetic analysis revealed that Gabonese DENV-3 originated from West African strains and has been circulating continuously in Gabon since at least 2010, when the first DENV-3 case was reported. CONCLUSIONS: These findings indicate stable DENV-3 circulation and the risk of repeated DENV infections in Gabon, highlighting the need for continuous monitoring to control DENV infections.
Authors: Haruka Abe; Yuri Ushijima; Rodrigue Bikangui; Georgelin N Ondo; Vahid R Zadeh; Christelle M Pemba; Patrick I Mpingabo; Yui Igasaki; Sophia G de Vries; Martin P Grobusch; Marguerite M Loembe; Selidji T Agnandji; Bertrand Lell; Jiro Yasuda Journal: J Viral Hepat Date: 2020-06-29 Impact factor: 3.728
Authors: Yuri Ushijima; Haruka Abe; Georgelin Nguema Ondo; Rodrigue Bikangui; Marguerite Massinga Loembé; Vahid R Zadeh; Joseph G E Essimengane; Armel V N Mbouna; Emmanuel B Bache; Selidji T Agnandji; Bertrand Lell; Jiro Yasuda Journal: BMC Infect Dis Date: 2021-03-17 Impact factor: 3.090
Authors: Khalid Eltom; Khalid Enan; Abdel Rahim M El Hussein; Isam M Elkhidir Journal: Front Cell Infect Microbiol Date: 2021-05-25 Impact factor: 5.293