Wencheng Zhang1, Zhouyong Gao2, Guang Zeng3, Hui Xie4, Jinbo Liu5, Ning Liu6, Guangshun Wang7. 1. Department of oncology, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: zhangwencheng19841@163.com. 2. Department of oncology, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: gaozhouyong@163.com. 3. Department of thoracic surgery, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: zengguang@163.com. 4. Department of thoracic surgery, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: xiehui@163.com. 5. Department of oncology, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: liujinbo@163.com. 6. Department of pathology, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: liuning@163.com. 7. Department of oncology, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China. Electronic address: wgsmed@163.com.
Abstract
BACKGROUND: Urinary proteins could be useful as markers for the detection of non-small-cell lung cancer (NSCLC). We investigated the levels of two different proteins in urine samples from NSCLC patients and assessed their diagnostic value. METHODS: Urinary plasminogen (PLG) and fibrinogen gamma chain (FGG) levels in 112 NSCLC patients and 197 controls were detected using enzyme linked immunosorbent assay (ELISA). The expression of FGG and PLG in 20 NSCLC tissues and paired adjacent non-tumour tissues were detected through immunohistochemistry. The diagnostic value of FGG and PLG for NSCLC was evaluated through a receiver operating characteristic curve (ROC). RESULTS: PLG and FGG were significantly elevated in NSCLC tissues vs paired adjacent non-tumour tissues (p = 0.000) and in urinary samples from NSCLC patients vs healthy controls (p = 0.000). The expression level of PLG in urinary samples was related only to the histological type (p = 0.001). Further, ROC curve analysis revealed that PLG, FGG, and their combination could distinguish NSCLC and its subtypes from healthy controls with an AUC ranging from 0.827 to 0. 947. By comparing urine samples with matching plasma CEA from NSCLC stage I-IV patients (n = 81) and healthy controls (n = 31), the combination of CEA with PLG or FGG showed that the AUC was 0.889 and 0.806, respectively, which is superior to a single biomarker alone. CONCLUSIONS: These two urinary proteins could serve as potential markers for the diagnosis of NSCLC.
BACKGROUND: Urinary proteins could be useful as markers for the detection of non-small-cell lung cancer (NSCLC). We investigated the levels of two different proteins in urine samples from NSCLCpatients and assessed their diagnostic value. METHODS: Urinary plasminogen (PLG) and fibrinogen gamma chain (FGG) levels in 112 NSCLCpatients and 197 controls were detected using enzyme linked immunosorbent assay (ELISA). The expression of FGG and PLG in 20 NSCLC tissues and paired adjacent non-tumour tissues were detected through immunohistochemistry. The diagnostic value of FGG and PLG for NSCLC was evaluated through a receiver operating characteristic curve (ROC). RESULTS:PLG and FGG were significantly elevated in NSCLC tissues vs paired adjacent non-tumour tissues (p = 0.000) and in urinary samples from NSCLCpatients vs healthy controls (p = 0.000). The expression level of PLG in urinary samples was related only to the histological type (p = 0.001). Further, ROC curve analysis revealed that PLG, FGG, and their combination could distinguish NSCLC and its subtypes from healthy controls with an AUC ranging from 0.827 to 0. 947. By comparing urine samples with matching plasma CEA from NSCLC stage I-IV patients (n = 81) and healthy controls (n = 31), the combination of CEA with PLG or FGG showed that the AUC was 0.889 and 0.806, respectively, which is superior to a single biomarker alone. CONCLUSIONS: These two urinary proteins could serve as potential markers for the diagnosis of NSCLC.
Authors: Eda G Ramirez-Valles; Alicia Rodríguez-Pulido; Marcelo Barraza-Salas; Isaac Martínez-Velis; Iván Meneses-Morales; Víctor M Ayala-García; Carlos A Alba-Fierro Journal: Technol Cancer Res Treat Date: 2020 Jan-Dec