Literature DB >> 31816179

Laboratory Cultivation and Storage of Shigella.

Shelley M Payne1.   

Abstract

Shigella species, which are closely related to Escherichia coli, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid.
© 2019 by John Wiley & Sons, Inc. Basic Protocol 1: Growth of S. flexneri from frozen stocks or agar stabs Basic Protocol 2: Growth of S. flexneri in rich liquid medium Alternate Protocol 1: Growth of S. flexneri in rich defined medium Alternate Protocol 2: Growth of S. flexneri in minimal medium Basic Protocol 3: Storage of S. flexneri in frozen stocks Alternate Protocol 3: Storage of S. flexneri in agar stabs. © 2019 John Wiley & Sons, Inc.

Entities:  

Keywords:  Shigella; Shigella flexneri; bacterial cultivation

Mesh:

Substances:

Year:  2019        PMID: 31816179      PMCID: PMC7274091          DOI: 10.1002/cpmc.93

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  17 in total

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Journal:  Microbiol Rev       Date:  1991-06

2.  Investigating the Relatedness of Enteroinvasive Escherichia coli to Other E. coli and Shigella Isolates by Using Comparative Genomics.

Authors:  Tracy H Hazen; Susan R Leonard; Keith A Lampel; David W Lacher; Anthony T Maurelli; David A Rasko
Journal:  Infect Immun       Date:  2016-07-21       Impact factor: 3.441

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Authors:  F C Neidhardt; P L Bloch; D F Smith
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

4.  Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.

Authors:  J Wei; M B Goldberg; V Burland; M M Venkatesan; W Deng; G Fournier; G F Mayhew; G Plunkett; D J Rose; A Darling; B Mau; N T Perna; S M Payne; L J Runyen-Janecky; S Zhou; D C Schwartz; F R Blattner
Journal:  Infect Immun       Date:  2003-05       Impact factor: 3.441

5.  Virulence plasmid instability in Shigella flexneri 2a is induced by virulence gene expression.

Authors:  R Schuch; A T Maurelli
Journal:  Infect Immun       Date:  1997-09       Impact factor: 3.441

6.  Plasmid-mediated invasiveness of "Shigella-like" Escherichia coli.

Authors:  P J Sansonetti; H d'Hauteville; S B Formal; M Toucas
Journal:  Ann Microbiol (Paris)       Date:  1982 May-Jun

7.  Detection and differentiation of iron-responsive avirulent mutants on Congo red agar.

Authors:  S M Payne; R A Finkelstein
Journal:  Infect Immun       Date:  1977-10       Impact factor: 3.441

8.  Loss of pigmentation in Shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid.

Authors:  A T Maurelli; B Blackmon; R Curtiss
Journal:  Infect Immun       Date:  1984-01       Impact factor: 3.441

9.  Role of intracellular carbon metabolism pathways in Shigella flexneri virulence.

Authors:  E A Waligora; C R Fisher; N J Hanovice; A Rodou; E E Wyckoff; S M Payne
Journal:  Infect Immun       Date:  2014-04-14       Impact factor: 3.441

10.  Genome sequence of Shigella flexneri serotype 5a strain M90T Sm.

Authors:  Naoko T Onodera; Julie Ryu; Tanja Durbic; Corey Nislow; John M Archibald; John R Rohde
Journal:  J Bacteriol       Date:  2012-06       Impact factor: 3.490

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