Jia Liu1,2, Sheng Li2, Xiaojing Cheng1,2, Peng DU2, Yong Yang3, Wen G Jiang4. 1. Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K. 2. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Urological Surgery, Peking University Cancer Hospital and Institute, Beijing, P.R. China. 3. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Urological Surgery, Peking University Cancer Hospital and Institute, Beijing, P.R. China jiangw@cardiff.ac.uk yoya_urology@sina.com. 4. Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K. jiangw@cardiff.ac.uk yoya_urology@sina.com.
Abstract
BACKGROUND/AIM: The present study aimed to investigate the role of Homebox B2 (HOXB2) in bladder cancer (BC). MATERIALS AND METHODS: The Cancer Genome Atlas (TCGA) dataset was used to analyse HOXB2 expression in BC. The influence of HOXB2 on the cellular functions of BC cells was determined in both HOXB2 knockdown and HOXB2 overexpressed BC cell lines using in vitro assays. RESULTS: HOXB2 mRNA was significantly upregulated in luminal infiltrated and luminal papillary subtypes of BC. Drug Metabolism Cytochrome P450 was significantly enriched in BCs expressing high levels of HOXB2. Knockdown of HOXB2 from EJ138 cells reduced growth, adhesion and invasion. In contrast, overexpression of HOXB2 in RT112 cells induced growth and adhesion of bladder cancer cells. CONCLUSION: Increased HOXB2 expression in papillary BC can promote cell growth and adhesion of BC cells. Drug Metabolism Cytochrome P450 pathway was enriched in BCs overexpressing HOXB2. Copyright
BACKGROUND/AIM: The present study aimed to investigate the role of Homebox B2 (HOXB2) in bladder cancer (BC). MATERIALS AND METHODS: The Cancer Genome Atlas (TCGA) dataset was used to analyse HOXB2 expression in BC. The influence of HOXB2 on the cellular functions of BC cells was determined in both HOXB2 knockdown and HOXB2 overexpressed BC cell lines using in vitro assays. RESULTS:HOXB2 mRNA was significantly upregulated in luminal infiltrated and luminal papillary subtypes of BC. Drug MetabolismCytochrome P450 was significantly enriched in BCs expressing high levels of HOXB2. Knockdown of HOXB2 from EJ138 cells reduced growth, adhesion and invasion. In contrast, overexpression of HOXB2 in RT112 cells induced growth and adhesion of bladder cancer cells. CONCLUSION: Increased HOXB2 expression in papillary BC can promote cell growth and adhesion of BC cells. Drug MetabolismCytochrome P450 pathway was enriched in BCs overexpressing HOXB2. Copyright