BACKGROUND: This study aimed to evaluate the performance of modified carbapenem inactivation method (mCIM) combined EDTA-carbapenem inactivation method (eCIM), and inhibitor-based combined disk test (CDT) in the detection and distinguishing of carbapenemase production in Enterobacteriaceae. METHODS: A total of 101 nonrepetitive carbapenem insensitive Enterobacteriaceae [minimal inhibitory concentration (MIC) ≥2 µg/mL] were tested by mCIM, eCIM and CDT respectively, and the major carbapenemase genes including blaKPC, blaNDM, blaIMP, blaVIM and blaOXA-48-like genes were detected by polymerase chain reaction (PCR) as control. RESULTS: Seventy-nine (78.2%) of isolates were found to harbour one or more carbapenemase genes by PCR, with blaKPC and blaNDM being the most common genes. OXA-48-like genes were undetectable. The coincidence rate of mCIM combined eCIM and CDT was 97.5% (77/79) and 96.2% (76/79) respectively, compared with gene detection. Both assays had a misclassification in two blaKPC+NDM-producing isolates of Klebsiella oxytoca. The sensitivity and specificity of two assays above were 100.0% vs. 95.0% and 98.4% vs. 98.4%, respectively in distinguishing serine-carbapenemase, while they were 95.1% vs. 97.6% and 100% vs. 100.0%, respectively in distinguishing metallo-carbapenemase. CONCLUSIONS: mCIM combined eCIM and the CDT are both useful tools for the reliable detection and distinguishing single serine-carbapenemase or metallo-carbapenemase, but not for mixed types. 2019 Annals of Translational Medicine. All rights reserved.
BACKGROUND: This study aimed to evaluate the performance of modified carbapenem inactivation method (mCIM) combined EDTA-carbapenem inactivation method (eCIM), and inhibitor-based combined disk test (CDT) in the detection and distinguishing of carbapenemase production in Enterobacteriaceae. METHODS: A total of 101 nonrepetitive carbapenem insensitive Enterobacteriaceae [minimal inhibitory concentration (MIC) ≥2 µg/mL] were tested by mCIM, eCIM and CDT respectively, and the major carbapenemase genes including blaKPC, blaNDM, blaIMP, blaVIM and blaOXA-48-like genes were detected by polymerase chain reaction (PCR) as control. RESULTS: Seventy-nine (78.2%) of isolates were found to harbour one or more carbapenemase genes by PCR, with blaKPC and blaNDM being the most common genes. OXA-48-like genes were undetectable. The coincidence rate of mCIM combined eCIM and CDT was 97.5% (77/79) and 96.2% (76/79) respectively, compared with gene detection. Both assays had a misclassification in two blaKPC+NDM-producing isolates of Klebsiella oxytoca. The sensitivity and specificity of two assays above were 100.0% vs. 95.0% and 98.4% vs. 98.4%, respectively in distinguishing serine-carbapenemase, while they were 95.1% vs. 97.6% and 100% vs. 100.0%, respectively in distinguishing metallo-carbapenemase. CONCLUSIONS: mCIM combined eCIM and the CDT are both useful tools for the reliable detection and distinguishing single serine-carbapenemase or metallo-carbapenemase, but not for mixed types. 2019 Annals of Translational Medicine. All rights reserved.
Entities:
Keywords:
EDTA-carbapenem inactivation method (eCIM); Modified carbapenem inactivation method (mCIM); carbapenemase-producing Enterobacteriaceae (CPE); combined disk test (CDT)
Authors: Elizabeth B Hirsch; Kai-Tai Chang; Paola C Zucchi; Dennis N Francoeur; Kimberly R Ledesma; Vincent H Tam; Todd M Lasco Journal: J Infect Chemother Date: 2013-12-11 Impact factor: 2.211
Authors: David M Livermore; Danièle Meunier; Katie L Hopkins; Michel Doumith; Robert Hill; Rachel Pike; Peter Staves; Neil Woodford Journal: J Antimicrob Chemother Date: 2018-03-01 Impact factor: 5.790
Authors: Baharak Babouee Flury; Matthew J Ellington; Katie L Hopkins; Jane F Turton; Michel Doumith; Richard Loy; Peter Staves; Vladimira Hinic; Reno Frei; Neil Woodford Journal: Antimicrob Agents Chemother Date: 2016-03-25 Impact factor: 5.191
Authors: Samar S Mabrouk; Ghada R Abdellatif; Mona R El-Ansary; Khaled M Aboshanab; Yasser M Ragab Journal: Infect Drug Resist Date: 2020-09-11 Impact factor: 4.003