Zhifeng Fang1,2, Lingzhi Li1,2, Jianxian Zhao1,2,3, Hao Zhang1,2,4,5,3, Yuan-Kun Lee6, Wenwei Lu7,8,9,10, Wei Chen1,2,4,11. 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China. 2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China. 3. (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, China. 4. National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, 214122, Jiangsu, China. 5. Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institut Wuxi Branch, Wuxi, 214122, Jiangsu, China. 6. Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. 7. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China. luwenwei@jiangnan.edu.cn. 8. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China. luwenwei@jiangnan.edu.cn. 9. National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, 214122, Jiangsu, China. luwenwei@jiangnan.edu.cn. 10. (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, China. luwenwei@jiangnan.edu.cn. 11. Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, China.
Abstract
PURPOSE: Emerging studies have reported gut microbial composition plays a key role in alleviating AD clinical symptoms during the probiotic intervention, but the correlation among clinical symptoms, immune responses and gut microbial alteration needs to be explored. Therefore, the objective was to investigate the correlation during Bifidobacterium adolescentis intervention in DNFB-induced AD mice. METHODS: The mice were randomly divided into nine groups and fed B. adolescentis for 3 weeks. At the end of the experiment, clinical and immune indicators were assessed. Flow cytometry was performed to explore the effect of B. adolescentis on regulatory T cells in the spleen. V3-V4 region of the 16S ribosomal RNA (rRNA) gene was sequenced to evaluate changes in the gut microbiota. RESULTS: Bifidobacteria adolescentis treatments reduced ear and skin thickness and suppressed eosinophils and mast cells infiltration. Th1- and Th2-type responses were regulated and the Tregs population was promoted in the spleen by B. adolescentis treatments. Bifidobacteria adolescentis increased the relative abundance of Lactobacillus but decrease Dorea and Pediococcus. Propionic and butyric acids were increased but isovaleric acid was decreased by B. adolescentis treatment. Besides, the functional modules, such as fatty acid biosynthesis, antigen processing and presentation were upregulated by B. adolescentis Ad1 treatment compared to the DNFB group. CONCLUSION: Collectively, these results imply that B. adolescentis with the role of immunomodulation promotes Treg differentiation and suppresses Th2 responses, and increases the proportion of Lactobacillus that is positively correlated to increase in propionic acid production, and thus has the potential for AD amelioration.
PURPOSE: Emerging studies have reported gut microbial composition plays a key role in alleviating AD clinical symptoms during the probiotic intervention, but the correlation among clinical symptoms, immune responses and gut microbial alteration needs to be explored. Therefore, the objective was to investigate the correlation during Bifidobacterium adolescentis intervention in DNFB-induced AD mice. METHODS: The mice were randomly divided into nine groups and fed B. adolescentis for 3 weeks. At the end of the experiment, clinical and immune indicators were assessed. Flow cytometry was performed to explore the effect of B. adolescentis on regulatory T cells in the spleen. V3-V4 region of the 16S ribosomal RNA (rRNA) gene was sequenced to evaluate changes in the gut microbiota. RESULTS: Bifidobacteria adolescentis treatments reduced ear and skin thickness and suppressed eosinophils and mast cells infiltration. Th1- and Th2-type responses were regulated and the Tregs population was promoted in the spleen by B. adolescentis treatments. Bifidobacteria adolescentis increased the relative abundance of Lactobacillus but decrease Dorea and Pediococcus. Propionic and butyric acids were increased but isovaleric acid was decreased by B. adolescentis treatment. Besides, the functional modules, such as fatty acid biosynthesis, antigen processing and presentation were upregulated by B. adolescentis Ad1 treatment compared to the DNFB group. CONCLUSION: Collectively, these results imply that B. adolescentis with the role of immunomodulation promotes Treg differentiation and suppresses Th2 responses, and increases the proportion of Lactobacillus that is positively correlated to increase in propionic acid production, and thus has the potential for AD amelioration.
Authors: Majda Dzidic; Thomas R Abrahamsson; Alejandro Artacho; Bengt Björkstén; Maria Carmen Collado; Alex Mira; Maria C Jenmalm Journal: J Allergy Clin Immunol Date: 2016-08-13 Impact factor: 10.793
Authors: Donald Y M Leung; Mark Boguniewicz; Michael D Howell; Ichiro Nomura; Qutayba A Hamid Journal: J Clin Invest Date: 2004-03 Impact factor: 14.808
Authors: K Reginald; K Westritschnig; T Werfel; A Heratizadeh; N Novak; M Focke-Tejkl; A M Hirschl; D Y M Leung; O Elisyutina; E Fedenko; R Valenta Journal: Clin Exp Allergy Date: 2010-12-14 Impact factor: 5.018
Authors: Jacob Mashiah; Tal Karady; Naomi Fliss-Isakov; Eli Sprecher; Dan Slodownik; Ofir Artzi; Liat Samuelov; Eran Ellenbogen; Anastasia Godneva; Eran Segal; Nitsan Maharshak Journal: Immun Inflamm Dis Date: 2021-12-20
Authors: Selina R Cox; Hazel Clarke; Majella O'Keeffe; Patrick Dubois; Peter M Irving; James O Lindsay; Kevin Whelan Journal: J Crohns Colitis Date: 2021-12-18 Impact factor: 9.071