BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory skin disease affecting up to 20% children and 9% adults world-wide. AD patients are often sensitized against a broad variety of allergens and more than 90% of them suffer from skin superinfections with Staphylococcus aureus. OBJECTIVE: In this study, we searched for the presence of specific IgE antibodies against S. aureus and Escherichia coli antigens in AD patients. METHODS: Sera from AD patients (n=79), patients suffering only from allergic rhinoconjunctivitis (n=41) or allergic asthma (n=37) were tested for IgE reactivity to nitrocellulose-blotted S. aureus, E. coli and gut bacterial antigens. IgE-reactive bacterial antigens were affinity purified and identified by mass spectrometry. RESULTS: More than 30% of AD patients but not patients suffering only from allergic rhinoconjunctivitis and asthma or non-allergic persons exhibited IgE binding to several protein antigens among them DNA-binding and ribosomal proteins and flagellin. Patients with severe skin manifestations showed more frequently IgE reactivity to S. aureus compared with AD patients with mild symptoms. Positive immediate and late skin test reactions could be induced in sensitized AD patients with S. aureus extract. CONCLUSION AND CLINICAL RELEVANCE: Specific IgE reactivities against a variety of bacterial antigens were observed in a subgroup comprising a third of AD patients and may contribute to allergic inflammation.
BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory skin disease affecting up to 20% children and 9% adults world-wide. AD patients are often sensitized against a broad variety of allergens and more than 90% of them suffer from skin superinfections with Staphylococcus aureus. OBJECTIVE: In this study, we searched for the presence of specific IgE antibodies against S. aureus and Escherichia coli antigens in AD patients. METHODS: Sera from AD patients (n=79), patients suffering only from allergic rhinoconjunctivitis (n=41) or allergic asthma (n=37) were tested for IgE reactivity to nitrocellulose-blotted S. aureus, E. coli and gut bacterial antigens. IgE-reactive bacterial antigens were affinity purified and identified by mass spectrometry. RESULTS: More than 30% of AD patients but not patients suffering only from allergic rhinoconjunctivitis and asthma or non-allergic persons exhibited IgE binding to several protein antigens among them DNA-binding and ribosomal proteins and flagellin. Patients with severe skin manifestations showed more frequently IgE reactivity to S. aureus compared with AD patients with mild symptoms. Positive immediate and late skin test reactions could be induced in sensitized AD patients with S. aureus extract. CONCLUSION AND CLINICAL RELEVANCE: Specific IgE reactivities against a variety of bacterial antigens were observed in a subgroup comprising a third of AD patients and may contribute to allergic inflammation.
Authors: D Y Leung; R Harbeck; P Bina; R F Reiser; E Yang; D A Norris; J M Hanifin; H A Sampson Journal: J Clin Invest Date: 1993-09 Impact factor: 14.808
Authors: Z Su; J Lin; F Lu; X Zhang; L Zhang; N B Gandhi; C S de Paiva; S C Pflugfelder; D-Q Li Journal: Mucosal Immunol Date: 2013-01-09 Impact factor: 7.313
Authors: Ruzhi Deng; Zhitao Su; Fan Lu; Lili Zhang; Jing Lin; Xiaobo Zhang; Cintia S de Paiva; Stephen C Pflugfelder; De-Quan Li Journal: Exp Eye Res Date: 2014-01-29 Impact factor: 3.467
Authors: Julia K Gittler; Avner Shemer; Mayte Suárez-Fariñas; Judilyn Fuentes-Duculan; Kara J Gulewicz; Claire Q F Wang; Hiroshi Mitsui; Irma Cardinale; Cristina de Guzman Strong; James G Krueger; Emma Guttman-Yassky Journal: J Allergy Clin Immunol Date: 2012-08-27 Impact factor: 10.793
Authors: Eric L Simpson; Miguel Villarreal; Brett Jepson; Nick Rafaels; Gloria David; Jon Hanifin; Patricia Taylor; Mark Boguniewicz; Takeshi Yoshida; Anna De Benedetto; Kathleen C Barnes; Donald Y M Leung; Lisa A Beck Journal: J Invest Dermatol Date: 2018-03-28 Impact factor: 8.551
Authors: Raffaela Campana; Sheron Dzoro; Irene Mittermann; Elena Fedenko; Olga Elisyutina; Musa Khaitov; Alexander Karaulov; Rudolf Valenta Journal: Curr Opin Allergy Clin Immunol Date: 2017-08