| Literature DB >> 31776874 |
Guohui Xiao1,2, Su Zhang1, Zhihang Liang3, Guanqiang Li4, Mutong Fang1, Yaya Liu1, Juanjuan Zhang1, Min Ou1, Xing He1, Tianyu Zhang2, Changchun Zeng5, Lei Liu1, Guoliang Zhang6,7.
Abstract
The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify M. abscessus species and subspecies. By designing specific sgRNA probes targeting rpoB and erm(41), we demonstrated that M. abscessus could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical M. abscessus isolates were identified, 18 as M. abscessus subsp. abscessus and 20 as M. abscessus subsp. massiliense. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of M. abscessus species and subspecies.Entities:
Keywords: FnCas12a; Identification; Mycobacterium abscessus subsp. abscessus; Mycobacterium abscessus subsp. bolletii; Mycobacterium abscessus subsp. massiliense; sgRNA probe
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Year: 2019 PMID： 31776874 DOI： 10.1007/s10096-019-03757-y
Source DB: PubMed Journal: Eur J Clin Microbiol Infect Dis ISSN： 0934-9723 Impact factor: 3.267