Arif Aslam1, Sumaira Z Aasi2. 1. Oxford Dermatology, Mount Hawthorn, Perth, Western Australia. 2. Department of Dermatology, Stanford University, Redwood City, California.
Abstract
BACKGROUND: Mohs micrographic surgery (MMS) is the most reliable tissue-sparing technique in the management of cutaneous malignancies. Although the concept is simple, there is considerable variability in the mapping and processing techniques used by Mohs surgeons and histotechnicians. OBJECTIVE: This review article aims to examine the frozen-section tissue processing techniques. Existing variations will be discussed and pearls offered to optimize the frozen processing technique. METHODS: A PubMed search was performed for publications on methods of tissue processing in MMS. RESULTS: Our review highlights variations in debulking, embedding, processing adipose tissue, cartilage, and wedge resections. We offer pearls on how to avoid false-positive and false-negative margins and discuss advances in immunohistochemistry. CONCLUSION: Our article provides a how-to format on the different stages of tissue processing with pearls and techniques to optimize practice and improve accuracy.
BACKGROUND: Mohs micrographic surgery (MMS) is the most reliable tissue-sparing technique in the management of cutaneous malignancies. Although the concept is simple, there is considerable variability in the mapping and processing techniques used by Mohs surgeons and histotechnicians. OBJECTIVE: This review article aims to examine the frozen-section tissue processing techniques. Existing variations will be discussed and pearls offered to optimize the frozen processing technique. METHODS: A PubMed search was performed for publications on methods of tissue processing in MMS. RESULTS: Our review highlights variations in debulking, embedding, processing adipose tissue, cartilage, and wedge resections. We offer pearls on how to avoid false-positive and false-negative margins and discuss advances in immunohistochemistry. CONCLUSION: Our article provides a how-to format on the different stages of tissue processing with pearls and techniques to optimize practice and improve accuracy.
Authors: Marc Combalia; Sergio Garcia; Josep Malvehy; Susana Puig; Alba Guembe Mülberger; James Browning; Sandra Garcet; James G Krueger; Samantha R Lish; Rivka Lax; Jeannie Ren; Mary Stevenson; Nicole Doudican; John A Carucci; Manu Jain; Kevin White; Jaroslav Rakos; Daniel S Gareau Journal: Biomed Opt Express Date: 2021-05-05 Impact factor: 3.562