| Literature DB >> 31762469 |
Luther M Swift1, Rafael Jaimes1, Damon McCullough1, Morgan Burke1, Marissa Reilly1, Takuya Maeda2, Hanyu Zhang3, Nobuyuki Ishibashi2, Jack M Rogers3, Nikki Gillum Posnack4.
Abstract
Small animal models are most commonly used in cardiovascular research due to the availability of genetically modified species and lower cost compared to larger animals. Yet, larger mammals are better suited for translational research questions related to normal cardiac physiology, pathophysiology, and preclinical testing of therapeutic agents. To overcome the technical barriers associated with employing a larger animal model in cardiac research, we describe an approach to measure physiological parameters in an isolated, Langendorff-perfused piglet heart. This approach combines two powerful experimental tools to evaluate the state of the heart: electrophysiology (EP) study and simultaneous optical mapping of transmembrane voltage and intracellular calcium using parameter sensitive dyes (RH237, Rhod2-AM). The described methodologies are well suited for translational studies investigating the cardiac conduction system, alterations in action potential morphology, calcium handling, excitation-contraction coupling and the incidence of cardiac alternans or arrhythmias.Entities:
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Year: 2019 PMID： 31762469 PMCID： PMC7597439 DOI： 10.3791/60472
Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355